The release of Extracellular Vesicles (EVs) into the bloodstream is positively associated with Particulate Matter (PM) exposure, which is involved in endothelial dysfunction and related to increased risk of cardiovascular disease. Obesity modifies the effects of PM exposure on heart rate variability and markers of inflammation, oxidative stress, and acute phase response. We isolated and characterized plasmatic EVs from six healthy donors and confirmed a positive association with PM exposure. We stratified for Body Mass Index (BMI) and observed an increased release of CD61+ (platelets) and CD105+ (endothelium) derived-EVs after high PM level exposure in Normal Weight subjects (NW) and no significant variations in Overweight subjects (OW). We then investigated the ability to activate endothelial primary cells by plasmatic EVs after both high and low PM exposure. NW-high-PM EVs showed an increased endothelial activation, measured as CD105+/CD62e+ (activated endothelium) EVs ratio. On the contrary, cells treated with OW-high-PM EVs showed reduced endothelial activation. These results suggest the ability of NW plasmatic EVs to communicate to endothelial cells and promote the crosstalk between activated endothelium and peripheral cells. However, this capacity was lost in OW subjects. Our findings contribute to elucidate the role of EVs in endothelial activation after PM exposure.
Blood-derived extracellular vesicles isolated from healthy donors exposed to air pollution modulate in vitro endothelial cells behavior / F. Rota, L. Ferrari, M. Hoxha, C. Favero, R. Antonioli, L. Pergoli, M.F. Greco, J. Mariani, L. Lazzari, V. Bollati. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 10:1(2020 Nov 18).
|Titolo:||Blood-derived extracellular vesicles isolated from healthy donors exposed to air pollution modulate in vitro endothelial cells behavior|
ROTA, FEDERICA (Co-primo)
FERRARI, LUCA (Co-primo)
HOXHA, MIRJAM (Secondo)
BOLLATI, VALENTINA (Ultimo) [Funding Acquisition] (Corresponding)
|Settore Scientifico Disciplinare:||Settore MED/44 - Medicina del Lavoro|
|Progetto:||INSIDE project: INdividual air pollution exposure, extracellular vesicle SIgnaling and hypertensive disorder DEvelopment in pregnancy.|
Susceptibily to Particle Healt Effects, miRNAs and Exosomes
|Data di pubblicazione:||18-nov-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41598-020-77097-9|
|Appare nelle tipologie:||01 - Articolo su periodico|