The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O < Stack >(2) < Superscript >-supercript stop </Stack>-generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.
Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances Paraquat-induced photooxidative stress and Nitric Oxide-induced cell death / D. Tarantino, C. Vannini, M. Bracale, M. Campa, C. Soave, I. Murgia. - In: PLANTA. - ISSN 0032-0935. - 221:6(2005), pp. 757-765.
|Titolo:||Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances Paraquat-induced photooxidative stress and Nitric Oxide-induced cell death|
TARANTINO, DELIA (Primo)
SOAVE, CARLO (Penultimo)
MURGIA, IRENE (Ultimo)
|Parole Chiave:||antisense; Arabidopsis thaliana; ascorbate peroxidase; nitric oxide; oxidative stress; chloroplasts|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00425-005-1485-9|
|Appare nelle tipologie:||01 - Articolo su periodico|