In feline species, cooled transport of ovaries can be employed without detrimental effects to retrieve immature oocytes intended for in vitro embryo production purposes. Indeed, this is the most common way to collect gametes from gonads of wild, valuable animals after they die or are castrated far from specialized laboratories. However, fresh retrieved gametes are generally used,and their cryosensitivity is not known. This study employed ovariectomy-derived domestic cat gonads as a model for wild felids, and aimed to compare the yield and developmental competence of Cryotop-vitrified oocytes (VOs) collected and cryopreserved right after ovary excision (In loco34 VOs) or after 24 hours cooled transport of ovaries (Shipped-VOs). The number of collected oocytes was higher in In loco-VOs than in Shipped-VOs (mean ± SD: 8 ± 3.36 vs 5.6 ± 3.1, p=0.05). In vitro embryo production resulted in similar maturation (35% for both vitrified groups, p=1) and fertilization rates (In loco-VOs: 29.1%; Shipped-VOs: 22.2%; p=0.295), but showed a in cleavage (In loco-VOs: 25.6%; Shipped-VOs: 14.5%; p=0.0495). No differences were found in further embryo development. Taken together, results suggested that delayed oocyte vitrification after cooled transport of organs was feasible and allowed embryo development. However, the number of collected oocytes and the cleavage rate of matured oocytes were higher when oocyte vitrification was performed without delay after ovary excision, and this should be considered in gamete conservation programs for endangered felids.
Ovary cold storage and shipment affect oocyte yield and cleavage rate of cat immature vitrified oocytes / M. Colombo, J. Zahmel, C. Binder, J. Herbel, G.C. Luvoni, K. Jewgenow. - In: CRYOBIOLOGY. - ISSN 0011-2240. - 98(2021 Feb), pp. 181-186.
Ovary cold storage and shipment affect oocyte yield and cleavage rate of cat immature vitrified oocytes
M. Colombo
Primo
;G.C. LuvoniPenultimo
;
2021
Abstract
In feline species, cooled transport of ovaries can be employed without detrimental effects to retrieve immature oocytes intended for in vitro embryo production purposes. Indeed, this is the most common way to collect gametes from gonads of wild, valuable animals after they die or are castrated far from specialized laboratories. However, fresh retrieved gametes are generally used,and their cryosensitivity is not known. This study employed ovariectomy-derived domestic cat gonads as a model for wild felids, and aimed to compare the yield and developmental competence of Cryotop-vitrified oocytes (VOs) collected and cryopreserved right after ovary excision (In loco34 VOs) or after 24 hours cooled transport of ovaries (Shipped-VOs). The number of collected oocytes was higher in In loco-VOs than in Shipped-VOs (mean ± SD: 8 ± 3.36 vs 5.6 ± 3.1, p=0.05). In vitro embryo production resulted in similar maturation (35% for both vitrified groups, p=1) and fertilization rates (In loco-VOs: 29.1%; Shipped-VOs: 22.2%; p=0.295), but showed a in cleavage (In loco-VOs: 25.6%; Shipped-VOs: 14.5%; p=0.0495). No differences were found in further embryo development. Taken together, results suggested that delayed oocyte vitrification after cooled transport of organs was feasible and allowed embryo development. However, the number of collected oocytes and the cleavage rate of matured oocytes were higher when oocyte vitrification was performed without delay after ovary excision, and this should be considered in gamete conservation programs for endangered felids.File | Dimensione | Formato | |
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