Dendritic cells (DCs) play a crucial role in the complex interplay between tumor cells and the immune system. During the elimination phase of cancer immunoediting, immunostimulatory DCs are critical for the control of tumor growth. During the escape phase, regulatory DCs sustain tumor tolerance and contribute to the development of the immunosuppressive tumor microenvironment that characterizes this phase. Moreover, increasing evidence indicates that DCs are also critical for the success of cancer immunotherapy. Hence, there is increasing need to fully characterize DC subsets and their activatory/inhibitory profile in cancer patients. In this review, we describe the role played by different DC subsets in the different phases of cancer immunoediting, the function exerted by different activatory and inhibitory molecules expressed on DC surface, and the cytokines produced by distinct DC subsets, in order to provide an overview on the DC features that may be useful to be assessed when dealing with the flow cytometric characterization of DCs in cancer patients.

Comprehensive phenotyping of dendritic cells in cancer patients by flow cytometry / C. Carenza, S. Franzese, F. Calcaterra, D. Mavilio, S.A.M. Della Bella. - In: CYTOMETRY. PART A. - ISSN 1552-4922. - 99:3(2021 Mar), pp. 218-230. [10.1002/cyto.a.24245]

Comprehensive phenotyping of dendritic cells in cancer patients by flow cytometry

C. Carenza;S. Franzese;F. Calcaterra;D. Mavilio;S.A.M. Della Bella
2021-03

Abstract

Dendritic cells (DCs) play a crucial role in the complex interplay between tumor cells and the immune system. During the elimination phase of cancer immunoediting, immunostimulatory DCs are critical for the control of tumor growth. During the escape phase, regulatory DCs sustain tumor tolerance and contribute to the development of the immunosuppressive tumor microenvironment that characterizes this phase. Moreover, increasing evidence indicates that DCs are also critical for the success of cancer immunotherapy. Hence, there is increasing need to fully characterize DC subsets and their activatory/inhibitory profile in cancer patients. In this review, we describe the role played by different DC subsets in the different phases of cancer immunoediting, the function exerted by different activatory and inhibitory molecules expressed on DC surface, and the cytokines produced by distinct DC subsets, in order to provide an overview on the DC features that may be useful to be assessed when dealing with the flow cytometric characterization of DCs in cancer patients.
human DCs; conventional DCs (cDCs); plasmacytoid DCs (pDCs); peripheral blood DCs (PBDCs); tumor‐ associated DCs (TADCs); cancer immunoediting; costimulatory molecules; immune checkpoints; flow cytometry
Settore MED/04 - Patologia Generale
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
24-ott-2020
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/778555
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