Bacterial adhesion onto abiotic surfaces is an important issue in biology and medicine since understanding the bases of such interaction represents a crucial aspect in the design of safe implant devices with intrinsic antibacterial characteristics. In this framework, we investigated the effects of nanostructured metal substrates on Escherichia coli adhesion and adaptation in order to understand the bio-molecular dynamics ruling the interactions at the interface. In particular, we show how highly controlled nanostructured gold substrates impact the bacterial behavior in terms of morphological changes and lead to modifications in the expression profile of several genes, which are crucially involved in the stress response and fimbrial synthesis. These results mainly demonstrate that E. coli cells are able to sense even slight changes in surface nanotopography and to actively respond by activating stress-related pathways. At the same time, our findings highlight the possibility of designing nanoengineered substrates able to trigger specific bio-molecular effects, thus opening the perspective of smartly tuning bacterial behavior by biomaterial design.
Molecular response of Escherichia coli adhering onto nanoscale topography / L. Rizzello, A. Galeone, G. Vecchio, V. Brunetti, S. Sabella, P.P. Pompa. - In: NANOSCALE RESEARCH LETTERS. - ISSN 1931-7573. - 7:1(2012), pp. 575.1-575.8.
|Titolo:||Molecular response of Escherichia coli adhering onto nanoscale topography|
|Parole Chiave:||Bacteria; Nanotopography; Adhesion; Molecular response|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
Settore BIO/14 - Farmacologia
|Data di pubblicazione:||2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1186/1556-276X-7-575|
|Appare nelle tipologie:||01 - Articolo su periodico|