The development of nutraceutical ingredients has risen as a nutritional solution for health prevention. This study evaluated the effects of Oleactiv®, an ingredient developed for the prevention of atherogenesis, in hypercholesterolemic hamsters. Oleactiv® is a polyphenol-rich ingredient obtained from artichoke, olive and grape extracts as part of fruit and vegetables commonly consumed within the Mediterranean diet. A total of 21 Golden Syrian hamsters were divided into three groups. The standard group (STD) was fed a normolipidemic diet for 12 weeks, while the control group (CTRL) and Oleactiv® goup (OLE) were fed a high-fat diet. After sacrifice, the aortic fatty streak area (AFSA), plasmatic total cholesterol (TC), high-density lipoproteins (HDL-C), non-HDL-C and triglycerides (TG), were assessed. The cholesterol efflux capacity (CEC) of hamster plasma was quantified using a radiolabeled technique in murine macrophages J774. OLE administration induced a significant reduction of AFSA (−69%, p < 0.0001). Hamsters of the OLE group showed a significant decrease of both non-HDL-C (−173 mmol/L, p < 0.05) and TG (−154 mmol/L, p < 0.05). Interestingly, OLE induced a significant increase of total CEC (+17,33%, p < 0,05). Oleactiv® supplementation prevented atheroma development and had positive effects on the lipid profile of hypercholesterolemic hamsters. The increased CEC underlines the anti-atherosclerotic mechanism at the root of the atheroma reduction observed.

Anti-atherosclerotic effect of a polyphenol-rich ingredient, oleactiv®, in a hypercholesterolemia-induced golden syrian hamster model / C. Romain, A. Piemontese, S. Battista, F. Bernini, A. Ossoli, A. Strazzella, S. Gaillet, J.-. Rouanet, J. Cases, I. Zanotti. - In: NUTRIENTS. - ISSN 2072-6643. - 10:10(2018 Oct 15), pp. 1511.1-1511.13. [10.3390/nu10101511]

Anti-atherosclerotic effect of a polyphenol-rich ingredient, oleactiv®, in a hypercholesterolemia-induced golden syrian hamster model

A. Ossoli;A. Strazzella;
2018-10-15

Abstract

The development of nutraceutical ingredients has risen as a nutritional solution for health prevention. This study evaluated the effects of Oleactiv®, an ingredient developed for the prevention of atherogenesis, in hypercholesterolemic hamsters. Oleactiv® is a polyphenol-rich ingredient obtained from artichoke, olive and grape extracts as part of fruit and vegetables commonly consumed within the Mediterranean diet. A total of 21 Golden Syrian hamsters were divided into three groups. The standard group (STD) was fed a normolipidemic diet for 12 weeks, while the control group (CTRL) and Oleactiv® goup (OLE) were fed a high-fat diet. After sacrifice, the aortic fatty streak area (AFSA), plasmatic total cholesterol (TC), high-density lipoproteins (HDL-C), non-HDL-C and triglycerides (TG), were assessed. The cholesterol efflux capacity (CEC) of hamster plasma was quantified using a radiolabeled technique in murine macrophages J774. OLE administration induced a significant reduction of AFSA (−69%, p < 0.0001). Hamsters of the OLE group showed a significant decrease of both non-HDL-C (−173 mmol/L, p < 0.05) and TG (−154 mmol/L, p < 0.05). Interestingly, OLE induced a significant increase of total CEC (+17,33%, p < 0,05). Oleactiv® supplementation prevented atheroma development and had positive effects on the lipid profile of hypercholesterolemic hamsters. The increased CEC underlines the anti-atherosclerotic mechanism at the root of the atheroma reduction observed.
Aortic fatty streak; Artichoke; Atheroma plaque; Atherosclerosis; Cholesterol efflux capacity; Grape; High fat diet; Olive; Polyphenol; Reverse cholesterol transport; Animals; Anticholesteremic Agents; Aorta, Thoracic; Aortic Diseases; Atherosclerosis; Cell Line; Cholesterol; Cholesterol, HDL; Diet, High-Fat; Disease Models, Animal; Hypercholesterolemia; Macrophages; Male; Mesocricetus; Mice; Plaque, Atherosclerotic; Polyphenols; Triglycerides; Dietary Supplements
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/770240
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