Croton rhamnifolioides is used in popular medicine for the treatment of inflammatory diseases. The objective of this study was to characterize and evaluate the anti-inflammatory effect of C. rhamnifolioides essential oil complexed in β-cyclodextrin (COEFC). The physicochemical characterization of the complexes was performed using different physical methods. The anti-inflammatory activity was evaluated in vivo by ear edema, paw edema, cotton pellet-induced granuloma, and vascular permeability by Evans blue extravasation. The mechanism of action was validated by molecular docking of the major constituent into the cyclooxygenase-2 (COX-2 enzyme). All doses of the COEFC reduced acute paw edema induced by carrageenan and dextran, as well as vascular permeability. Our results suggest the lowest effective dose of all samples inhibited the response induced by histamine or arachidonic acid as well as the granuloma formation. The complexation process showed that the pharmacological effects were maintained, however, showing similar results using much lower doses. The results demonstrated an involvement of the inhibition of pathways dependent on eicosanoids and histamine. Complexation of β-cyclodextrin/Essential oil (β-CD/EO) may present an important tool in the study of new compounds for the development of anti-inflammatory drugs.

Anti-inflammatory and physicochemical characterization of the croton rhamnifolioides essential oil inclusion complex in β-cyclodextrin / A.O.B.P.B. Martins, A.G. Wanderley, I.S. Alcantara, L.B. Rodrigues, F.R.A.S. Cesario, M.R.C. de Oliveira, F.F. E Castro, T.R. de Albuquerque, M.S.A. da Silva, J. Ribeiro-Filho, H.D.M. Coutinho, P.P. Menezes, L.J. Quintans-Junior, A.A.S. Araujo, M. Iriti, J.R.G.D.S. Almeida, I.R.A. de Menezes. - In: BIOLOGY. - ISSN 2079-7737. - 9:6(2020 Jun), pp. 114.1-114.25. [10.3390/biology9060114]

Anti-inflammatory and physicochemical characterization of the croton rhamnifolioides essential oil inclusion complex in β-cyclodextrin

M. Iriti;
2020

Abstract

Croton rhamnifolioides is used in popular medicine for the treatment of inflammatory diseases. The objective of this study was to characterize and evaluate the anti-inflammatory effect of C. rhamnifolioides essential oil complexed in β-cyclodextrin (COEFC). The physicochemical characterization of the complexes was performed using different physical methods. The anti-inflammatory activity was evaluated in vivo by ear edema, paw edema, cotton pellet-induced granuloma, and vascular permeability by Evans blue extravasation. The mechanism of action was validated by molecular docking of the major constituent into the cyclooxygenase-2 (COX-2 enzyme). All doses of the COEFC reduced acute paw edema induced by carrageenan and dextran, as well as vascular permeability. Our results suggest the lowest effective dose of all samples inhibited the response induced by histamine or arachidonic acid as well as the granuloma formation. The complexation process showed that the pharmacological effects were maintained, however, showing similar results using much lower doses. The results demonstrated an involvement of the inhibition of pathways dependent on eicosanoids and histamine. Complexation of β-cyclodextrin/Essential oil (β-CD/EO) may present an important tool in the study of new compounds for the development of anti-inflammatory drugs.
Croton rhamnifolioides; Cyclodextrin; Inflammation
Settore AGR/12 - Patologia Vegetale
giu-2020
Article (author)
File in questo prodotto:
File Dimensione Formato  
biology-09-00114-v2.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 2.42 MB
Formato Adobe PDF
2.42 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/769764
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 12
social impact