1. Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.

Effect of serotonin on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis / M. Garcia-Gil, D. Bottai, M.L. Zaccardi, M. Cannizzaro, M. Brunelli. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. C, COMPARATIVE PHARMACOLOGY AND TOXICOLOGY. - ISSN 0742-8413. - 104:1(1993), pp. 125-131.

Effect of serotonin on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis

D. Bottai
Secondo
;
1993

Abstract

1. Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.
aplysia sensory neurons ; Kinase-C ; segmental ganglia ; cyclic-AMP ; culture ; 5-hydroxytrtptamine ; hyperpolarization ; facilitation ; transmitter
Settore BIO/09 - Fisiologia
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
Settore BIO/14 - Farmacologia
1993
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/7652
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