A correct magnesium (Mg2+) intake is essential for bone health. In particular, Mg2+ deficiency inhibits the proliferation of osteoblast-like SaOS-2 cells by increasing nitric oxide (NO) production through the upregulation of inducible NO synthase. At the moment, little is known about the expression and the role of TRPM7, a channel/enzyme involved in Mg2+ uptake, and MagT1, a Mg2+ selective transporter, in SaOS-2 cells. Here, we demonstrate that TRPM7 is not modulated by different extracellular concentrations of Mg2+ and its silencing exacerbates growth inhibition exerted by low Mg2+ through the activation of inducible NO synthase and consequent accumulation of NO. Moreover, MagT1 is upregulated in SaOS-2 cultured in high Mg2+ and its silencing inhibits the growth of SaOS-2 cultured in media containing physiological or high Mg2+, without any modulation of NO production. We propose that TRPM7 and MagT1 are both involved in regulating SaOS-2 proliferation through different mechanisms.
TRPM7 and MagT1 regulate the proliferation of osteoblast-like SaOS-2 cells through different mechanisms / M. Zocchi, R. Scrimieri, L. Locatelli, A. Cazzaniga, G. Fedele, J.A.M. Maier, S. Castiglioni. - In: MAGNESIUM RESEARCH. - ISSN 0953-1424. - 33:1(2020 Mar), pp. 12-20.
|Titolo:||TRPM7 and MagT1 regulate the proliferation of osteoblast-like SaOS-2 cells through different mechanisms|
ZOCCHI, MONICA (Primo)
SCRIMIERI, ROBERTA (Secondo)
MAIER, JEANETTE ANNE MARIE (Penultimo)
CASTIGLIONI, SARA (Ultimo) (Corresponding)
|Parole Chiave:||bone; Magnesium; MagT1; osteoblasts; TRPM7|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||mar-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1684/mrh.2020.0463|
|Appare nelle tipologie:||01 - Articolo su periodico|