Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine endowed with a luminescent Ru complex, Ru-PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artefacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use polyamidoamineruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus.

Light-triggered trafficking to the cell nucleus of a cationic polyamidoamine functionalized with ruthenium complexes / L. Mascheroni, V. Francia, B. Rossotti, E. Ranucci, P. Ferruti, D. Maggioni, A. Salvati. - In: ACS APPLIED MATERIALS & INTERFACES. - ISSN 1944-8244. - (2020). [Epub ahead of print]

Light-triggered trafficking to the cell nucleus of a cationic polyamidoamine functionalized with ruthenium complexes

B. Rossotti;E. Ranucci;P. Ferruti;D. Maggioni
Penultimo
;
2020

Abstract

Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine endowed with a luminescent Ru complex, Ru-PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artefacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use polyamidoamineruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus.
polyamidoamines, luminescent ruthenium complexes, nuclear trafficking, endosomal escape, light-triggered escape
Settore CHIM/03 - Chimica Generale e Inorganica
Settore CHIM/04 - Chimica Industriale
2020
9-lug-2020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/753640
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