Creatinine in human serum was separated in a fused-silica capillary with H3PO4 (75 mmol/L, pH 2.5) as BGE, followed by UV detection at 200 nm. Serum with methylimidazole added as internal standard was deproteinized with acetonitrile and the supernatant, after dilution with water was injected at pressure mode. Creatinine and methylimidazole were baseline-resolved in 6.5 min. Linearity in the 0–880 mmol/L range gave an r2 0.998, recovery was 102 6 2.8% (n = 6). Enzymatic breakdown with creatininase confirmed that serum does not interfere. The within-day and betweendays coefficient of variation (CV) were 2.16 and 2.7%, respectively. The accuracy, determined for lyophilized samples by isotope dilution gas chromatography-mass spectrometry was 6 2.0%. The results were compared with HPLC for 32 lyophilized samples and on 27 serum pools. Capillary electrophoresis, rapid and inexpensive, seems a promising alternative to high-performance liquid chromatography (HPLC) for creatinine determination in human serum.
Creatinine determination in serum by capillary electrophoresis / R. Paroni, I. Fermo, G. Cighetti, C.A. Ferrero, A. Carobene, F. Ceriotti. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 25:3(2004), pp. 463-468.
Creatinine determination in serum by capillary electrophoresis
R. ParoniPrimo
;G. Cighetti;
2004
Abstract
Creatinine in human serum was separated in a fused-silica capillary with H3PO4 (75 mmol/L, pH 2.5) as BGE, followed by UV detection at 200 nm. Serum with methylimidazole added as internal standard was deproteinized with acetonitrile and the supernatant, after dilution with water was injected at pressure mode. Creatinine and methylimidazole were baseline-resolved in 6.5 min. Linearity in the 0–880 mmol/L range gave an r2 0.998, recovery was 102 6 2.8% (n = 6). Enzymatic breakdown with creatininase confirmed that serum does not interfere. The within-day and betweendays coefficient of variation (CV) were 2.16 and 2.7%, respectively. The accuracy, determined for lyophilized samples by isotope dilution gas chromatography-mass spectrometry was 6 2.0%. The results were compared with HPLC for 32 lyophilized samples and on 27 serum pools. Capillary electrophoresis, rapid and inexpensive, seems a promising alternative to high-performance liquid chromatography (HPLC) for creatinine determination in human serum.
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