Speckle-typePOZ(poxvirusandzincﬁngerprotein)protein(SPOP)isthemostcommonly mutated gene in prostate cancer (PCa). Recent evidence reports a role of SPOP in DNA damage response(DDR),indicatingapossibleimpactofSPOPderegulationonPCaradiosensitivity. Thisstudy aimedtodeﬁnetheroleofSPOPderegulation(bygenemutationorknockdown)asaradiosensitizing factor in PCa preclinical models. To express WT or mutant (Y87N, K129E and F133V) SPOP, DU145 and PC-3 cells were transfected with pMCV6 vectors. Sensitivity proﬁles were assessed using clonogenic assay and immunoﬂuorescent staining of γH2AX and RAD51 foci. SCID xenografts were treated with 5 Gy single dose irradiation using an image-guided small animal irradiator. siRNA and miRNA mimics were used to silence SPOP or express the SPOP negative regulator miR-145, respectively. SPOP deregulation, by either gene mutation or knockdown, consistently enhanced the radiation response of PCa models by impairing DDR, as indicated by transcriptome analysis and functionally conﬁrmed by decreased RAD51 foci. SPOP silencing also resulted in a signiﬁcant downregulation of RAD51 and CHK1 expression, consistent with the impairment of homologous recombination. Our results indicate that SPOP deregulation plays a radiosensitizing role in PCa by impairing DDR via downregulation of RAD51 and CHK1.
SPOP deregulation improves the radiation response of prostate cancer models by impairing DNA damage repair / R.E. Bezawy, M. Tripari, S. Percio, A. Cicchetti, M. Tortoreto, C. Stucchi, S. Tinelli, V. Zuco, V. Doldi, P. Gandellini, R. Valdagni, N. Zaffaroni. - In: CANCERS. - ISSN 2072-6694. - 12:6(2020 Jun), pp. 1462.1-1462.15.
|Titolo:||SPOP deregulation improves the radiation response of prostate cancer models by impairing DNA damage repair|
|Parole Chiave:||Mutation; Prostate cancer; Radiosensitivity; SPOP|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||giu-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/cancers12061462|
|Appare nelle tipologie:||01 - Articolo su periodico|