Chromosomal rearrangements of the MYC locus, which often involve the IG loci, are recurrent events in multiple myeloma (MM) and plasma cell leukemia (PCL). We used dual-color fluorescence in situ hybridization (FISH) to characterize the breakpoint locations of chromosomal translocations/rearrangements involving the MYC locus at 8q24 found in a panel of 14 MM cell lines and 70 primary tumors (66 MM and 4 PCL). MYC locus alterations were observed in 21 cases: MYCIIG (mainly IGH@) fusions in II cell lines and three patients (2 MM and 1 PCL), and extra signals and/or abnormal MYC localizations in seven patients (5 MM and 2 PCL). Fourteen of these cases were investigated by FISH analyses by use of a panel of BAC clones covering about 6 Mb encompassing the MYC locus. The breakpoints were localized in a region 100-250 kb centromeric to MYC in four cases, a region 500-800 kb telomeric to the gene in four cases, and regions ≥2 Mb centromeric or telomeric to MYC in five cases. Two different breakpoints were detected in the KMS-18 cell line, whereas the insertion of a MYC allele was found in a complex t(16;22) chromosomal translocation in the RPM18226 cell line. Our data document a relatively high dispersion of 8q24 breakpoints in MM.

Heterogeneous pattern of chromosomal breakpoints involving the MYC locus in multiple myeloma / S. Fabris, C.T. Storlazzi, L. Baldini, L. Nobili, L. Lombardi, A.T. Maiolo, M. Rocchi, A. Neri. - In: GENES, CHROMOSOMES & CANCER. - ISSN 1045-2257. - 37:3(2003), pp. 261-269.

Heterogeneous pattern of chromosomal breakpoints involving the MYC locus in multiple myeloma

S. Fabris;L. Baldini;L. Nobili;A.T. Maiolo;A. Neri
2003

Abstract

Chromosomal rearrangements of the MYC locus, which often involve the IG loci, are recurrent events in multiple myeloma (MM) and plasma cell leukemia (PCL). We used dual-color fluorescence in situ hybridization (FISH) to characterize the breakpoint locations of chromosomal translocations/rearrangements involving the MYC locus at 8q24 found in a panel of 14 MM cell lines and 70 primary tumors (66 MM and 4 PCL). MYC locus alterations were observed in 21 cases: MYCIIG (mainly IGH@) fusions in II cell lines and three patients (2 MM and 1 PCL), and extra signals and/or abnormal MYC localizations in seven patients (5 MM and 2 PCL). Fourteen of these cases were investigated by FISH analyses by use of a panel of BAC clones covering about 6 Mb encompassing the MYC locus. The breakpoints were localized in a region 100-250 kb centromeric to MYC in four cases, a region 500-800 kb telomeric to the gene in four cases, and regions ≥2 Mb centromeric or telomeric to MYC in five cases. Two different breakpoints were detected in the KMS-18 cell line, whereas the insertion of a MYC allele was found in a complex t(16;22) chromosomal translocation in the RPM18226 cell line. Our data document a relatively high dispersion of 8q24 breakpoints in MM.
Settore MED/15 - Malattie del Sangue
2003
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/736881
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