Toll-Like Receptors (TLRs) play a critical role in the innate and adaptive immune system. They are the mammalian orthologs of Drosophila melanogaster protein Toll, which has been proved to have an early morphogenetic role in invertebrate embryogenesis that in the adult switches to an immune function. Aim: The aim of this study was to evaluate the expression of TLR4 and TLR7 during dorsal root ganglia (DRG), paravertebral ganglia (PVG), and enteric nervous system (ENS) murine development. Methods: Mouse embryos from different stages (i.e. E12 to E18) were processed for immunolocalization analysis on formalin-fixed paraffin-embedded sections, and isolated intestine were processed for whole-mount preparations. Results: We observed a differentially regulated expression of TLR4 and TLR7 during embryogenesis and an overall increased expression of both receptors during development. While TLR4 was detectable in neurons of DRG and PVG starting from E14 and only from E18 in the ENS, TLR7 was already expressed in scattered neurons of all the investigated regions at E12. Conclusions: TLR4 and TRL7 expression temporal patterns suggest a morphogenetic role for these receptors in the development of neural crest derivatives in mammals.

Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development / F. Arnaboldi, M. Sommariva, E. Opizzi, M. Rasile, S. Camelliti, M. Busnelli, E. Menegola, F. Di Renzo, A. Menon, I. Barajon. - In: ANNALS OF ANATOMY. - ISSN 0940-9602. - 231(2020 Sep). [10.1016/j.aanat.2020.151526]

Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development

F. Arnaboldi
Primo
;
M. Sommariva
Secondo
;
E. Opizzi;M. Rasile;S. Camelliti;M. Busnelli;E. Menegola;F. Di Renzo;A. Menon
Penultimo
;
2020

Abstract

Toll-Like Receptors (TLRs) play a critical role in the innate and adaptive immune system. They are the mammalian orthologs of Drosophila melanogaster protein Toll, which has been proved to have an early morphogenetic role in invertebrate embryogenesis that in the adult switches to an immune function. Aim: The aim of this study was to evaluate the expression of TLR4 and TLR7 during dorsal root ganglia (DRG), paravertebral ganglia (PVG), and enteric nervous system (ENS) murine development. Methods: Mouse embryos from different stages (i.e. E12 to E18) were processed for immunolocalization analysis on formalin-fixed paraffin-embedded sections, and isolated intestine were processed for whole-mount preparations. Results: We observed a differentially regulated expression of TLR4 and TLR7 during embryogenesis and an overall increased expression of both receptors during development. While TLR4 was detectable in neurons of DRG and PVG starting from E14 and only from E18 in the ENS, TLR7 was already expressed in scattered neurons of all the investigated regions at E12. Conclusions: TLR4 and TRL7 expression temporal patterns suggest a morphogenetic role for these receptors in the development of neural crest derivatives in mammals.
Toll-like receptors; development; immunolocalization; peripheral nervous system; whole-mount
Settore BIO/16 - Anatomia Umana
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/14 - Farmacologia
set-2020
4-mag-2020
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/735918
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