Staphylococcus aureus is the most important etiologic agent of contagious mastitis able to produce a number of virulence factors that may play important roles in the development of this disease. Recently, a rapid technique based on the amplification of the 16S-23S rRNA intergenic space (RS-PCR) was developed for S. aureus genotyping The aim of the present study was to characterize certain strains collected from North Italy herds to verify the presence of a specific genotype associated in Switzerland with severe clinical expression in the herd. In the 30 strains analyzed, our preliminary results showed the presence of four isolates similar to Switzerland genotype B and the possibility to link an electrophoretic profile to a specific pattern of virulence.
Staphylococcus aureus: Application of a rapid test for molecular typing of strains isolated from bovine mastitis / P. Cremonesi, V. Benedetti, G. Pisoni, P. Moroni, M. Luini, B. Castiglioni - In: Veterinary Science : Current Aspects in Biology, Animal Pathology, Clinic and Food Hygiene / [a cura di] A. Pugliese, A. Gaiti, C. Boiti. - Berlin : Springer-Verlag, 2014. - ISBN 9783642232701. - pp. 41-45 [10.1007/978-3-642-23271-8_7]
Staphylococcus aureus: Application of a rapid test for molecular typing of strains isolated from bovine mastitis
G. Pisoni;P. Moroni;
2014
Abstract
Staphylococcus aureus is the most important etiologic agent of contagious mastitis able to produce a number of virulence factors that may play important roles in the development of this disease. Recently, a rapid technique based on the amplification of the 16S-23S rRNA intergenic space (RS-PCR) was developed for S. aureus genotyping The aim of the present study was to characterize certain strains collected from North Italy herds to verify the presence of a specific genotype associated in Switzerland with severe clinical expression in the herd. In the 30 strains analyzed, our preliminary results showed the presence of four isolates similar to Switzerland genotype B and the possibility to link an electrophoretic profile to a specific pattern of virulence.
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