Reduced blood flow, as occurring in ischemia or resulting from exposure to microgravity such as encountered in space flights, induces a decrease in the level of shear stress sensed by endothelial cells forming the inner part of blood vessels. In the present study, we use a microvasculature-on-a-chip device in order to investigate in vitro the effect of such a reduction in shear stress on shear-adapted endothelial cells. We find that, within 1 h of exposition to reduced wall shear stress, human umbilical vein endothelial cells undergo reorganization of their actin skeleton with a decrease in the number of stress fibers and actin being recruited into the cells' peripheral band, indicating a fairly fast change in the cells' phenotype due to altered flow.
The effect of shear stress reduction on endothelial cells : a microfluidic study of the actin cytoskeleton / M. Inglebert, L. Locatelli, D. Tsvirkun, K.P. Sinha, J.A. Maier, C. Misbah, L. Bureau. - In: BIOMICROFLUIDICS. - ISSN 1932-1058. - 14:2(2020 Apr).
Titolo: | The effect of shear stress reduction on endothelial cells : a microfluidic study of the actin cytoskeleton |
Autori: | |
Settore Scientifico Disciplinare: | Settore MED/04 - Patologia Generale |
Data di pubblicazione: | apr-2020 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1063/1.5143391 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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BMF19-AR-00412.pdf | Post-print, accepted manuscript ecc. (versione accettata dall'editore) | Open Access Visualizza/Apri |