Reduced blood flow, as occurring in ischemia or resulting from exposure to microgravity such as encountered in space flights, induces a decrease in the level of shear stress sensed by endothelial cells forming the inner part of blood vessels. In the present study, we use a microvasculature-on-a-chip device in order to investigate in vitro the effect of such a reduction in shear stress on shear-adapted endothelial cells. We find that, within 1 h of exposition to reduced wall shear stress, human umbilical vein endothelial cells undergo reorganization of their actin skeleton with a decrease in the number of stress fibers and actin being recruited into the cells' peripheral band, indicating a fairly fast change in the cells' phenotype due to altered flow.
The effect of shear stress reduction on endothelial cells : a microfluidic study of the actin cytoskeleton / M. Inglebert, L. Locatelli, D. Tsvirkun, K.P. Sinha, J.A. Maier, C. Misbah, L. Bureau. - In: BIOMICROFLUIDICS. - ISSN 1932-1058. - 14:2(2020 Apr).
|Titolo:||The effect of shear stress reduction on endothelial cells : a microfluidic study of the actin cytoskeleton|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||apr-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1063/1.5143391|
|Appare nelle tipologie:||01 - Articolo su periodico|