Interleukin 17 (IL-17)-producing T helper cells (TH-17 cells) have been characterized in mice as a distinct subset of effector cells, but their identity and properties in humans remain elusive. We report here that expression of CCR6 and CCR4 together identified human memory CD4+T cells selectively producing IL-17 and expressing mRNA encoding the human ortholog of mouse ROR gamma t, a transcription factor, whereas CCR6 and CXCR3 identified T(H)1 cells producing interferon-c and T helper cells producing both interferon-c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+ CCR4+ TH-17 subset, whereas memory T cells specific for Mycobacterium tuberculosis were present in CCR6+ CXCR3+ T helper type 1 subset. The elicitation of IL-17 responses correlated with the capacity of C. albicans hyphae to stimulate antigen-presenting cells for the priming of TH-17 responses in vitro and for the production of IL-23 but not IL-12. Our results demonstrate that human TH-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.
Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells / E. Acosta-Rodriguez, L. Rivino, J. Geginat, D. Jarrossay, M. Gattorno, A. Lanzavecchia, F. Sallusto, G. Napolitani. - In: NATURE IMMUNOLOGY. - ISSN 1529-2908. - 8:6(2007), pp. 639-646.
Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells
J. Geginat;
2007
Abstract
Interleukin 17 (IL-17)-producing T helper cells (TH-17 cells) have been characterized in mice as a distinct subset of effector cells, but their identity and properties in humans remain elusive. We report here that expression of CCR6 and CCR4 together identified human memory CD4+T cells selectively producing IL-17 and expressing mRNA encoding the human ortholog of mouse ROR gamma t, a transcription factor, whereas CCR6 and CXCR3 identified T(H)1 cells producing interferon-c and T helper cells producing both interferon-c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+ CCR4+ TH-17 subset, whereas memory T cells specific for Mycobacterium tuberculosis were present in CCR6+ CXCR3+ T helper type 1 subset. The elicitation of IL-17 responses correlated with the capacity of C. albicans hyphae to stimulate antigen-presenting cells for the priming of TH-17 responses in vitro and for the production of IL-23 but not IL-12. Our results demonstrate that human TH-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.File | Dimensione | Formato | |
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