Blastocystis is a ubiquitous protozoan with a wide range of hosts. In humans, its presence has been associated with gastrointestinal disorders, although its role as a pathogen still needs to be elucidated. Until now, 17 Blastocystis subtypes (STs) have been identified, with ST1–ST4 the most commonly found in humans. Among domestic animals, the same STs reported in humans have been detected in dogs. An epidemiological survey on dog kennels was carried out to evaluate the prevalence of Blastocystis and the STs involved. Overall, 99 faecal samples were collected from the rescue shelters. Blastocystis detection was performed through conventional barcoding PCR targeting the 1800-bp SSU-rDNA, followed by sequencing and phylogenetic analysis. Blastocystis DNA was found in 21 faecal samples (21.2%), and all samples were successfully sequenced and identified as ST3 in a unique monophyletic group. The presence of Blastocystis was reported for the first time in dogs from Italy, with the identification of ST3, the subtype most commonly found in humans.
Molecular epidemiology of Blastocystis sp. in dogs housed in Italian rescue shelters / A.L. Gazzonis, M. Marangi, S.A. Zanzani, L. Villa, A. Giangaspero, M.T. Manfredi. - In: PARASITOLOGY RESEARCH. - ISSN 0932-0113. - 118:10(2019 Oct), pp. 3011-3017.
|Titolo:||Molecular epidemiology of Blastocystis sp. in dogs housed in Italian rescue shelters|
GAZZONIS, ALESSIA LIBERA (Corresponding)
|Parole Chiave:||Blastocystis; Dog; Genotyping; Molecular epidemiology; Subtypes; Zoonosis; Animals; Blastocystis; Blastocystis Infections; DNA, Protozoan; DNA, Ribosomal; Dog Diseases; Dogs; Feces; Female; Italy; Male; Molecular Epidemiology; Phylogeny; Prevalence; Sequence Analysis, DNA|
|Settore Scientifico Disciplinare:||Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali|
|Data di pubblicazione:||ott-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00436-019-06424-5|
|Appare nelle tipologie:||01 - Articolo su periodico|
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