Peroxisome proliferator-activated receptor-γ (PPARγ) is essential for adipogenesis. Since EDF-1 is a cofactor of PPARγ, we investigated the molecular cross-talk between EDF-1 and PPARγ in adipogenesis. While EDF-1 was not modulated during differentiation of 3T3-L1 cells, it co-immunoprecipitated with PPARγ. Silencing EDF-1 by shRNAs inhibited the differentiation in adipocytes of 3T3-L1 cells, as detected by the staining of intracellular triglycerides and the expression of the PPARγ target gene aP2. Accordingly, we found that anti-EDF-1 shRNAs decreased ligand dependent activation of PPARγ in 3T3-L1 transiently transfected with a vector expressing luciferase under the control of a PPARγ responsive consensus. To rule out that this inhibition is due to the concomitant downregulation of PPARγ levels, we overexpressed PPARγ in 3T3-L1 silencing EDF-1 and found a decrease of ligand dependent activation of PPARγ, in spite of the high amounts of PPARγ. These results demonstrate that EDF-1 is required for PPARγ transcriptional activation during 3T3-L1 differentiation.
|Titolo:||Transcriptional coactivator EDF-1 is required for PPARγ-stimulated adipogenesis|
|Parole Chiave:||Adipogenesis; EDF-1; PPARγ; Transcriptional coactivator|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||10.1007/s00018-009-0069-4|
|Appare nelle tipologie:||01 - Articolo su periodico|