Background A mutation in the canine multidrug resistance MDR1 gene (also referred as ABCB1), encoding for the multidrug resistance (MDR) P-glycoprotein (P-gp) transponder, causes a pathological condition known as “ivermectine toxicosis”. The causative mutation, known since 2001, has been described to affects sheep herding breeds related to collie lineage. The present study is a retrospective investigation of the presence of MDR1 mutated allele in Italian dog populations in a five years’ time lapse. The aim of the research is to offer a deep knowledge in MDR1 allelic and genotypic frequencies in canine breeds and populations raised in Italy. Methods Genotype data for the 4 bp deletion (c296_299del4) in MDR1 gene from 811 dogs belonging to 32 breeds/populations were collected. Results The mutated allele has been found in 9 out of 31 breeds: Rough Collie, Smooth Collie, Border Collie, Bearded Collie, Shetland Sheepdog, Australian Shepherd, White Swiss Shepherd, Old English Sheepdog, Whippet and also in crossbreed. The breeds with the highest allelic mutation frequency are Smooth and Rough Collies with 75 and 66% of mutant MDR1 allele respectively. Conclusions The results support the usefulness of this genetic analysis to optimize medical care in dogs at risk of multidrug resistance and to create objective basis in breeding programs definition and in the risk evaluation in different breeds.

Genotypic and allelic frequencies of MDR1 gene in dogs in Italy / S.P. Marelli, M. Polli, S. Frattini, M. Cortellari, R. Rizzi, P. Crepaldi. - In: VETERINARY RECORD OPEN. - ISSN 2052-6113. - 2020(2020 Jun 24), pp. e000375.1-e000375.5. [10.1136/vetreco-2019-000375]

Genotypic and allelic frequencies of MDR1 gene in dogs in Italy

S.P. Marelli
Primo
;
M. Polli
Secondo
;
S. Frattini;M. Cortellari;R. Rizzi
Penultimo
;
P. Crepaldi
Ultimo
2020-06-24

Abstract

Background A mutation in the canine multidrug resistance MDR1 gene (also referred as ABCB1), encoding for the multidrug resistance (MDR) P-glycoprotein (P-gp) transponder, causes a pathological condition known as “ivermectine toxicosis”. The causative mutation, known since 2001, has been described to affects sheep herding breeds related to collie lineage. The present study is a retrospective investigation of the presence of MDR1 mutated allele in Italian dog populations in a five years’ time lapse. The aim of the research is to offer a deep knowledge in MDR1 allelic and genotypic frequencies in canine breeds and populations raised in Italy. Methods Genotype data for the 4 bp deletion (c296_299del4) in MDR1 gene from 811 dogs belonging to 32 breeds/populations were collected. Results The mutated allele has been found in 9 out of 31 breeds: Rough Collie, Smooth Collie, Border Collie, Bearded Collie, Shetland Sheepdog, Australian Shepherd, White Swiss Shepherd, Old English Sheepdog, Whippet and also in crossbreed. The breeds with the highest allelic mutation frequency are Smooth and Rough Collies with 75 and 66% of mutant MDR1 allele respectively. Conclusions The results support the usefulness of this genetic analysis to optimize medical care in dogs at risk of multidrug resistance and to create objective basis in breeding programs definition and in the risk evaluation in different breeds.
Dog; Ivermectine Toxicosis; MDR1; breeding management; genetic analysis
Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/724257
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