Background: Prior studies failed to detect significant association between hypoalbuminemia and small intestinal lesions. Hypothesis: Use of pictorial templates will enhance consistency of interpathologist interpretation and identification of intestinal lesions associated with hypoalbuminemia. Animals: Tissues from 62 dogs and 25 cats examined as clinical cases at 7 referral veterinary practices in 4 countries. Methods: Retrospective, observational study. Histopathology slides from sequential cases undergoing endoscopic biopsy were examined by 4 pathologists by pictorial templates. Changes for 9 microscopic features were recorded as normal, mild, moderate or severe, and 2- and 4-point scales were tested for consistency of interpretation. Logistic regression models determined odds ratios (OR) of histologic lesions being associated with hypoalbuminemia while k statistics determined agreement between pathologists on histologic lesions. Results: There was poor agreement (k= -0.013 to 0.3) between pathologists, and institution of origin of slides had effect (k = 1.0 for 3 of 4 lesions on slides from Institution 5) on agreement between pathologists on selected histologic features. Using 2 point as opposed to 4-point grading scale increased agreement between pathologists (maximum k = 0.69 using 4-point scale versus maximum k = 1.0 using 2-point scale). Significant association (P = .019– .04; 95% OR = 3.14–10.84) between lacteal dilation and hypoalbuminemia was found by 3 pathologists. Conclusions and Clinical Importance: Substantial inconsistency between pathologists remains despite use of pictorial template because of differences in slide processing. Distinguishing between mild and moderate lesions might be important source of the disagreement among pathologists.
Effect of Tissue Processing on Assessment of Endoscopic Intestinal Biopsies in Dogs and Cats / M.D. Willard, G.E. Moore, B.D. Denton, M.J. Day, J. Mansell, T. Bilzer, B. Wilcock, M. Gualtieri, D. Olivero, P. Lecoindre, D.C. Twedt, M.G. Collett, E.J. Hall, A.E. Jergens, J.W. Simpson, R.W. Else, R.J. Washabau. - In: JOURNAL OF VETERINARY INTERNAL MEDICINE. - ISSN 0891-6640. - 24:1(2010 Jan), pp. 84-89.
|Titolo:||Effect of Tissue Processing on Assessment of Endoscopic Intestinal Biopsies in Dogs and Cats|
|Parole Chiave:||Histology; Inflammatory bowel disease; Protein-losing enteropathy|
|Settore Scientifico Disciplinare:||Settore VET/09 - Clinica Chirurgica Veterinaria|
|Data di pubblicazione:||gen-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1939-1676.2009.0432.x|
|Appare nelle tipologie:||01 - Articolo su periodico|