We previously identified an extracellular matrix (ECM) gene expression pattern in breast cancer (BC), called ECM3, characterized by a high expression of genes encoding structural ECM proteins. Since ECM is reportedly implicated in response to therapy of BCs, the aim of this work is to investigate the prognostic and predictive value of ECM3 molecular classification in HER2-positive BCs. ECM3 resulted in a robust cluster that identified a subset of 25-37% of HER2-positive tumors with molecular aggressive features. ECM3 was significantly associated with worse prognosis in two datasets of HER2-positive BCs untreated with adjuvant therapy. Analyses carried out on two of our cohorts of patients treated or not with adjuvant trastuzumab showed association of ECM3 with worse prognosis only in patients not treated with trastuzumab. Moreover, investigating a dataset that includes gene profile data of tumors treated with neoadjuvant trastuzumab plus chemotherapy or chemotherapy alone, ECM3 was associated with increased pathological complete response if treated with trastuzumab. In the in vivo experiments, increased diffusion and trastuzumab activity were found in tumors derived from injection of HER2-positive cells with Matrigel that creates an ECM-rich tumor environment. Taken together, these results indicate that HER2-positive BCs classified as ECM3 have an aggressive phenotype but they are sensitive to trastuzumab treatment.
Extracellular matrix features discriminate aggressive HER2-positive breast cancer patients who benefit from trastuzumab treatment / I. Rybinska, M. Sandri, F. Bianchi, R. Orlandi, L. De Cecco, P. Gasparini, M. Campiglio, B. Paolini, L. Sfondrini, E. Tagliabue, T. Triulzi. - In: CELLS. - ISSN 2073-4409. - 9:2(2020 Feb 13), pp. 434.1-434.14.
|Titolo:||Extracellular matrix features discriminate aggressive HER2-positive breast cancer patients who benefit from trastuzumab treatment|
|Parole Chiave:||ECM3; HER2 positive breast cancer; collagen; extracellular matrix; gene expression; trastuzumab|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore MED/06 - Oncologia Medica
|Data di pubblicazione:||13-feb-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/cells9020434|
|Appare nelle tipologie:||01 - Articolo su periodico|