We have analyzed the role of actin polymerization in retinoic acid (RA)-induced HoxB transcription, which is mediated by the HoxB regulator Prep1. RA induction of the HoxB genes can be prevented by the inhibition of actin polymerization. Importantly, inhibition of actin polymerization specifically affects the transcription of inducible Hox genes, but not that of their transcriptional regulators, the RARs, nor of constitutively expressed, nor of actively transcribed Hox genes. RA treatment induces the recruitment to the HoxB2 gene enhancer of a complex composed of "elongating" RNAPII, Prep1, β-actin, and N-WASP as well as the accessory splicing components p54Nrb and PSF. We show that inhibition of actin polymerization prevents such recruitment. We conclude that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes.
Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization / C. Ferrai, G. Naum-Ongania, E. Longobardi, M. Palazzolo, A. Disanza, V.M. Diaz, M.P. Crippa, G. Scita, F. Blasi. - In: MOLECULAR BIOLOGY OF THE CELL. - ISSN 1059-1524. - 20:15(2009 Aug), pp. 3543-3551.
|Titolo:||Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||ago-2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1091/mbc.E09-02-0114|
|Appare nelle tipologie:||01 - Articolo su periodico|