These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) / A. Cossarizza, H.-. Chang, A. Radbruch, A. Acs, D. Adam, S. Adam-Klages, W.W. Agace, N. Aghaeepour, M. Akdis, M. Allez, L.N. Almeida, G. Alvisi, G. Anderson, I. Andra, F. Annunziato, A. Anselmo, P. Bacher, C.T. Baldari, S. Bari, V. Barnaba, J. Barros-Martins, L. Battistini, W. Bauer, S. Baumgart, N. Baumgarth, D. Baumjohann, B. Baying, M. Bebawy, B. Becher, W. Beisker, V. Benes, R. Beyaert, A. Blanco, D.A. Boardman, C. Bogdan, J.G. Borger, G. Borsellino, P.E. Boulais, J.A. Bradford, D. Brenner, R.R. Brinkman, A.E.S. Brooks, D.H. Busch, M. Buscher, T.P. Bushnell, F. Calzetti, G. Cameron, I. Cammarata, X. Cao, S.L. Cardell, S. Casola, M.A. Cassatella, A. Cavani, A. Celada, L. Chatenoud, P.K. Chattopadhyay, S. Chow, E. Christakou, L. Cicin-Sain, M. Clerici, F.S. Colombo, L. Cook, A. Cooke, A.M. Cooper, A.J. Corbett, A. Cosma, L. Cosmi, P.G. Coulie, A. Cumano, L. Cvetkovic, V.D. Dang, C. Dang-Heine, M.S. Davey, D. Davies, S. De Biasi, G. Del Zotto, G.V. Dela Cruz, M. Delacher, S. Della Bella, P. Dellabona, G. Deniz, M. Dessing, J.P. Di Santo, A. Diefenbach, F. Dieli, A. Dolf, T. Dorner, R.J. Dress, D. Dudziak, M. Dustin, C.-. Dutertre, F. Ebner, S.B.G. Eckle, M. Edinger, P. Eede, G.R.A. Ehrhardt, M. Eich, P. Engel, B. Engelhardt, A. Erdei, C. Esser, B. Everts, M. Evrard, C.S. Falk, T.A. Fehniger, M. Felipo-Benavent, H. Ferry, M. Feuerer, A. Filby, K. Filkor, S. Fillatreau, M. Follo, I. Forster, J. Foster, G.A. Foulds, B. Frehse, P.S. Frenette, S. Frischbutter, W. Fritzsche, D.W. Galbraith, A. Gangaev, N. Garbi, B. Gaudilliere, R.T. Gazzinelli, J. Geginat, W. Gerner, N.A. Gherardin, K. Ghoreschi, L. Gibellini, F. Ginhoux, K. Goda, D.I. Godfrey, C. Goettlinger, J.M. Gonzalez-Navajas, C.S. Goodyear, A. Gori, J.L. Grogan, D. Grummitt, A. Grutzkau, C. Haftmann, J. Hahn, H. Hammad, G. Hammerling, L. Hansmann, G. Hansson, C.M. Harpur, S. Hartmann, A. Hauser, A.E. Hauser, D.L. Haviland, D. Hedley, D.C. Hernandez, G. Herrera, M. Herrmann, C. Hess, T. Hofer, P. Hoffmann, K. Hogquist, T. Holland, T. Hollt, R. Holmdahl, P. Hombrink, J.P. Houston, B.F. Hoyer, B. Huang, F.-. Huang, J.E. Huber, J. Huehn, M. Hundemer, C.A. Hunter, W.Y.K. Hwang, A. Iannone, F. Ingelfinger, S.M. Ivison, H.-. Jack, P.K. Jani, B. Javega, S. Jonjic, T. Kaiser, T. Kalina, T. Kamradt, S.H.E. Kaufmann, B. Keller, S.L.C. Ketelaars, A. Khalilnezhad, S. Khan, J. Kisielow, P. Klenerman, J. Knopf, H.-. Koay, K. Kobow, J.K. Kolls, W.T. Kong, M. Kopf, T. Korn, K. Kriegsmann, H. Kristyanto, T. Kroneis, A. Krueger, J. Kuhne, C. Kukat, D. Kunkel, H. Kunze-Schumacher, T. Kurosaki, C. Kurts, P. Kvistborg, I. Kwok, J. Landry, O. Lantz, P. Lanuti, F. Larosa, A. Lehuen, S. LeibundGut-Landmann, M.D. Leipold, L.Y.T. Leung, M.K. Levings, A.C. Lino, F. Liotta, V. Litwin, Y. Liu, H.-. Ljunggren, M. Lohoff, G. Lombardi, L. Lopez, M. Lopez-Botet, A.E. Lovett-Racke, E. Lubberts, H. Luche, B. Ludewig, E. Lugli, S. Lunemann, H.T. Maecker, L. Maggi, O. Maguire, F. Mair, K.H. Mair, A. Mantovani, R.A. Manz, A.J. Marshall, A. Martinez-Romero, G. Martrus, I. Marventano, W. Maslinski, G. Matarese, A.V. Mattioli, C. Maueroder, A. Mazzoni, J. Mccluskey, M. Mcgrath, H.M. Mcguire, I.B. Mcinnes, H.E. Mei, F. Melchers, S. Melzer, D. Mielenz, S.D. Miller, K.H.G. Mills, H. Minderman, J. Mjosberg, J. Moore, B. Moran, L. Moretta, T.R. Mosmann, S. Muller, G. Multhoff, L.E. Munoz, C. Munz, T. Nakayama, M. Nasi, K. Neumann, L.G. Ng, A. Niedobitek, S. Nourshargh, G. Nunez, J.-. O'Connor, A. Ochel, A. Oja, D. Ordonez, A. Orfao, E. Orlowski-Oliver, W. Ouyang, A. Oxenius, R. Palankar, I. Panse, K. Pattanapanyasat, M. Paulsen, D. Pavlinic, L. Penter, P. Peterson, C. Peth, J. Petriz, F. Piancone, W.F. Pickl, S. Piconese, M. Pinti, A.G. Pockley, M.J. Podolska, Z. Poon, K. Pracht, I. Prinz, C.E.M. Pucillo, S.A. Quataert, L. Quatrini, K.M. Quinn, H. Radbruch, T.R.D.J. Radstake, S. Rahmig, H.-. Rahn, B. Rajwa, G. Ravichandran, Y. Raz, J.A. Rebhahn, D. Recktenwald, D. Reimer, C. Reis e Sousa, E.B.M. Remmerswaal, L. Richter, L.G. Rico, A. Riddell, A.M. Rieger, J.P. Robinson, C. Romagnani, A. Rubartelli, J. Ruland, A. Saalmuller, Y. Saeys, T. Saito, S. Sakaguchi, F. Sala-de-Oyanguren, Y. Samstag, S. Sanderson, I. Sandrock, A. Santoni, R.B. Sanz, M. Saresella, C. Sautes-Fridman, B. Sawitzki, L. Schadt, A. Scheffold, H.U. Scherer, M. Schiemann, F.A. Schildberg, E. Schimisky, A. Schlitzer, J. Schlosser, S. Schmid, S. Schmitt, K. Schober, D. Schraivogel, W. Schuh, T. Schuler, R. Schulte, A.R. Schulz, S.R. Schulz, C. Scotta, D. Scott-Algara, D.P. Sester, T.V. Shankey, B. Silva-Santos, A.K. Simon, K.M. Sitnik, S. Sozzani, D.E. Speiser, J. Spidlen, A. Stahlberg, A.M. Stall, N. Stanley, R. Stark, C. Stehle, T. Steinmetz, H. Stockinger, Y. Takahama, K. Takeda, L. Tan, A. Tarnok, G. Tiegs, G. Toldi, J. Tornack, E. Traggiai, M. Trebak, T.I.M. Tree, J. Trotter, J. Trowsdale, M. Tsoumakidou, H. Ulrich, S. Urbanczyk, W. van de Veen, M. van den Broek, E. van der Pol, S. Van Gassen, G. Van Isterdael, R.A.W. van Lier, M. Veldhoen, S. Vento-Asturias, P. Vieira, D. Voehringer, H.-. Volk, A. von Borstel, K. von Volkmann, A. Waisman, R.V. Walker, P.K. Wallace, S.A. Wang, X.M. Wang, M.D. Ward, K.A. Ward-Hartstonge, K. Warnatz, G. Warnes, S. Warth, C. Waskow, J.V. Watson, C. Watzl, L. Wegener, T. Weisenburger, A. Wiedemann, J. Wienands, A. Wilharm, R.J. Wilkinson, G. Willimsky, J.B. Wing, R. Winkelmann, T.H. Winkler, O.F. Wirz, A. Wong, P. Wurst, J.H.M. Yang, J. Yang, M. Yazdanbakhsh, L. Yu, A. Yue, H. Zhang, Y. Zhao, S.M. Ziegler, C. Zielinski, J. Zimmermann, A. Zychlinsky. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - 49:10(2019 Oct), pp. 1457-1973. [10.1002/eji.201970107]

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

S. Casola;M. Clerici;S. Della Bella;J. Geginat;A. Gori;F. Piancone;
2019

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Settore MED/04 - Patologia Generale
ott-2019
Article (author)
File in questo prodotto:
File Dimensione Formato  
eji.201970107-2.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 20.19 MB
Formato Adobe PDF
20.19 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/714394
Citazioni
  • ???jsp.display-item.citation.pmc??? 392
  • Scopus 756
  • ???jsp.display-item.citation.isi??? 456
social impact