Surface-activated chemical ionization (SACI) has been widely used in recent years for the analysis of different compounds (e.g. peptides, street drugs, amino acids). The main benefits of this technology are its high sensitivity and its effectiveness under different chromatographic conditions [i.e. ion exchange chromatography and reversed-phase (RP) chromatography]. Here we used SACI in conjunction with quadrupole time-of-flight mass spectrometry to analyze enterotoxin A, which is produced by Staphylococcus aureus, in milk matrix using both RP and ion exchange chromatographies. SACI had increased sensitivity as compared with electrospray ionization. Moreover, the higher quantitation efficiency of this technique, mainly in terms of limit of detection (0.01 ng/ml), limit of quantitation (0.05 ng/ml), linearity range (0.05-50 ng/ml), matrix effect, accuracy (intraday and interday accuracy errors were 9.2% and 10.3%, respectively) and precision (intraday and interday precision errors were 5.3% and 12.8%, respectively), is shown and discussed.
Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A / S. Cristoni, L. Zingaro, C. Canton, P. Cremonesi, B. Castiglioni, S. Morandi, M. Brasca, M. Luzzana, C. Battaglia. - In: JOURNAL OF MASS SPECTROMETRY. - ISSN 1076-5174. - 44:10(2009), pp. 1482-1488.
|Titolo:||Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A|
LUZZANA, MASSIMO ROBERTO (Penultimo)
BATTAGLIA, CRISTINA (Ultimo)
|Parole Chiave:||mass spectrometry ; milk ; staphylococcus ; chromatography|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/jms.1636|
|Appare nelle tipologie:||01 - Articolo su periodico|