Among the heterogeneous population of circulating hematopoietic and endothelial progenitors, we identified a subpopulation of CD133+ cells displaying myogenic properties. Unexpectedly, we observed the expression of the B-cell marker CD20 in blood-derived CD133+ stem cells. The CD20 antigen plays a role in the modulation of intracellular calcium homeostasis through signaling pathways activation. Several observations suggest that an increase in intracellular calcium concentration ([Ca2+]i) could be involved in the etiology of the Duchenne muscular dystrophy (DMD). Here, we show that a CD20-related signaling pathway able to induce an increase in [Ca2+]i is differently activated after brain derived neurotrophic factor (BDNF) stimulation of normal and dystrophic blood-derived CD133+ stem cells, supporting the assumption of a “CD20-related calcium impairment-affecting dystrophic cells. Presented findings represent the starting point toward the expansion of knowledge on pathways involved in the pathology of DMD and in the behavior of dystrophic blood-derived CD133+ stem cells.
CD20-related signaling pathway is differently activated in normal and dystrophic circulating CD133+ stem cells / D. Parolini, M.A. Meregalli, M. Belicchi, P. Razini, R. Lopa, B. Del Carlo, A. Farini, S. Maciotta, N. Bresolin, L. Porretti, M. Pellegrino, Y. Torrente. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 66:4(2009 Jan 20), pp. 697-710. [10.1007/s00018-009-8652-2]
CD20-related signaling pathway is differently activated in normal and dystrophic circulating CD133+ stem cells
D. Parolini;M.A. Meregalli;M. Belicchi;P. Razini;A. Farini;S. Maciotta;N. Bresolin;Y. Torrente
2009
Abstract
Among the heterogeneous population of circulating hematopoietic and endothelial progenitors, we identified a subpopulation of CD133+ cells displaying myogenic properties. Unexpectedly, we observed the expression of the B-cell marker CD20 in blood-derived CD133+ stem cells. The CD20 antigen plays a role in the modulation of intracellular calcium homeostasis through signaling pathways activation. Several observations suggest that an increase in intracellular calcium concentration ([Ca2+]i) could be involved in the etiology of the Duchenne muscular dystrophy (DMD). Here, we show that a CD20-related signaling pathway able to induce an increase in [Ca2+]i is differently activated after brain derived neurotrophic factor (BDNF) stimulation of normal and dystrophic blood-derived CD133+ stem cells, supporting the assumption of a “CD20-related calcium impairment-affecting dystrophic cells. Presented findings represent the starting point toward the expansion of knowledge on pathways involved in the pathology of DMD and in the behavior of dystrophic blood-derived CD133+ stem cells.File | Dimensione | Formato | |
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