N6-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant KiPA-DNA=4.4×103 M-1 was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.
Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study / RAJABI MEHDI, P. Signorelli, GORINCIOI ELENA, R. Ghidoni, SANTANIELLO ENZO. - In: DNA AND CELL BIOLOGY. - ISSN 1044-5498. - 29:11(2010), pp. 687-691.
|Titolo:||Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study|
RAJABI, MEHDI (Primo) [Membro del Collaboration Group]
SIGNORELLI, PAOLA (Secondo) [Membro del Collaboration Group]
GORINCIOI, ELENA [Membro del Collaboration Group]
GHIDONI, RICCARDO (Penultimo)
SANTANIELLO, ENZO (Ultimo)
|Parole Chiave:||apoptosis; N6-isopentenyladenosine; proliferation; cytokinins; component; damage; agent; assay|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1089/dna.2010.1073|
|Appare nelle tipologie:||01 - Articolo su periodico|