N6-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant KiPA-DNA=4.4×103 M-1 was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.

Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study / RAJABI MEHDI, P. Signorelli, GORINCIOI ELENA, R. Ghidoni, SANTANIELLO ENZO. - In: DNA AND CELL BIOLOGY. - ISSN 1044-5498. - 29:11(2010), pp. 687-691. [10.1089/dna.2010.1073]

Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study

M. Rajabi
Primo
Membro del Collaboration Group
;
P. Signorelli
Secondo
Membro del Collaboration Group
;
E. Gorincioi
Membro del Collaboration Group
;
R. Ghidoni
Penultimo
;
E. Santaniello
Ultimo
2010

Abstract

N6-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant KiPA-DNA=4.4×103 M-1 was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.
apoptosis; N6-isopentenyladenosine; proliferation; cytokinins; component; damage; agent; assay
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/707515
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