A previous high-performance liquid chromatographic determination of diamine oxidase activity suitable for tissue homogenates was modified in order to adapt it to plasma samples. Simple additional steps were introduced after the enzyme reaction and before the chromatographic separation, both according to the previous method. In this way the sensitivity and the reproducibility of the overall procedure was suitable for routine plasma diamine oxidase estimations.
Modified high-performance liquid chromatographic determination of diamine oxidase activity in plasma / P.A. Biondi, F. Ceciliani, C. Gandini, C. Lucarelli. - In: JOURNAL OF CHROMATOGRAPHY. - ISSN 0021-9673. - 507:C(1990), pp. 333-337. ((Intervento presentato al 13. convegno International symp on column liquid chromatography tenutosi a Stockholm nel 1989.
Modified high-performance liquid chromatographic determination of diamine oxidase activity in plasma
P.A. Biondi;F. Ceciliani;C. Gandini;
1990
Abstract
A previous high-performance liquid chromatographic determination of diamine oxidase activity suitable for tissue homogenates was modified in order to adapt it to plasma samples. Simple additional steps were introduced after the enzyme reaction and before the chromatographic separation, both according to the previous method. In this way the sensitivity and the reproducibility of the overall procedure was suitable for routine plasma diamine oxidase estimations.
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