Mucositis is a frequent problem after irradiation of the oral mucosa. To study the early effects of irradiation on the desmosomal adhesion complex, explants of keratinized oral mucosa were exposed to a single dose of 2 Gy gamma irradiation. Biopsies were obtained from the upper dental arch of nine young healthy non-smoking women undergoing minor oral surgery. The biopsies were incubated in a Transwell culture system and, after irradiation, fixed in formalin 24 h later. Morphometric measurements of epithelial thickness revealed that it was unaffected by exposure to ionizing rays. Immunofluorescence analysis of desmosomal cadherin expression (desmoglein 1/desmoglein 3) demonstrated that the distribution of desmoglein 1 was not affected, whereas the expression of desmoglein 3 decreased in the suprabasal layers of irradiated samples. It is suggested that this has consequences for the mechanical integrity of the mucosa and promotes the development of radiation mucositis.
A comparative morphological study of the response to ionizing rays in organotypic cultures of human skin and oral mucosa / E. Donetti, M. Bedoni, A. Gualerzi, P.Capone, G. Tartaglia, C. Sforza. - In: EXPERIMENTAL DERMATOLOGY. - ISSN 0906-6705. - 18(2009), pp. 1086-1086. ((Intervento presentato al 36. convegno Annual Meeting of Society for Cutaneous Ultrastructure Research tenutosi a Firenze nel 2009.
|Titolo:||A comparative morphological study of the response to ionizing rays in organotypic cultures of human skin and oral mucosa|
DONETTI, ELENA BIANCA (Primo)
BEDONI, MARZIA (Secondo)
TARTAGLIA, GIANLUCA MARTINO (Penultimo)
SFORZA, CHIARELLA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/16 - Anatomia Umana|
|Data di pubblicazione:||2009|
|Appare nelle tipologie:||01 - Articolo su periodico|