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EnglishEmbryos utilize oocyte-donated RNAs until they become capable of producing RNAs through embryonic genome activation (EGA). The sperm’s influence over pre-EGA RNA content of embryos remains unknown. Recent studies have revealed that sperm donate non-genomic components upon fertilization. Thus, sperm may also contribute to RNA presence in pre-EGA embryos. The first objective of this study was to investigate whether male fertility status is associated with the RNAs present in the bovine embryo prior to EGA. A total of 65 RNAs were found to be differentially expressed between 2–4 cell bovine embryos derived from high and low fertility sires. Expression patterns were confirmed for protein phosphatase 1 regulatory subunit 36 (PPP1R36) and ataxin 2 like (ATXN2L) in three new biological replicates. The knockdown of ATXN2L led to a 22.9% increase in blastocyst development. The second objective of this study was to characterize the parental origin of RNAs present in pre-EGA embryos. Results revealed 472 sperm-derived RNAs, 2575 oocyte-derived RNAs, 2675 RNAs derived from both sperm and oocytes, and 663 embryo-exclusive RNAs. This study uncovers an association of male fertility with developmentally impactful RNAs in 2–4 cell embryos. This study also provides an initial characterization of paternally-contributed RNAs to pre-EGA embryos. Furthermore, a subset of 2–4 cell embryo-specific RNAs was identified.
Characterization and functional roles of paternal RNAs in 2–4 cell bovine embryos / N. Gross, M.G. Strillacci, F. Penagaricano, H. Khatib. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 9:1(2019), pp. 20347.1-20347.9.
| Titolo: | Characterization and functional roles of paternal RNAs in 2–4 cell bovine embryos | |
| Autori: | ||
| Settore Scientifico Disciplinare: | Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico | |
| Data di pubblicazione: | 2019 | |
| Rivista: | ||
| Tipologia: | Article (author) | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1038/s41598-019-55868-3 | |
| Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 2019_Gross.pdf | Publisher's version/PDF | Open Access Visualizza/Apri |
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