Exposure to real or simulated microgravity is sensed as a stress by mammalian cells, which activate a complex adaptive response. In human primary endothelial cells, we have recently shown the sequential intervention of various stress proteins which are crucial to prevent apoptosis and maintain cell function. We here demonstrate that mitophagy contributes to endothelial adaptation to gravitational unloading. After 4 and 10 d of exposure to simulated microgravity in the rotating wall vessel, the amount of BCL2 interacting protein 3, a marker of mitophagy, is increased and, in parallel, mitochondrial content, oxygen consumption, and maximal respiratory capacity are reduced, suggesting the acquisition of a thrifty phenotype to meet the novel metabolic challenges generated by gravitational unloading. Moreover, we suggest that microgravity induced-disorganization of the actin cytoskeleton triggers mitophagy, thus creating a connection between cytoskeletal dynamics and mitochondrial content upon gravitational unloading.
Mitophagy contributes to endothelial adaptation to simulated microgravity / L. Locatelli, A. Cazzaniga, C. De Palma, S. Castiglioni, J. Maier. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 34:1(2020 Jan), pp. 1833-1845.
|Titolo:||Mitophagy contributes to endothelial adaptation to simulated microgravity|
MAIER, JEANETTE ANNE MARIE (Corresponding)
|Parole Chiave:||HUVEC; RWV; cytoskeleton; mitochondria|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||gen-2020|
|Data ahead of print / Data di stampa:||8-dic-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1096/fj.201901785RRR|
|Appare nelle tipologie:||01 - Articolo su periodico|