The imaging performances of multiphoton excitation and confocal laser scanning microscopy are herby considered: in typical experimental imaging conditions, a small finite amount of photon reaches the detector giving shot-noise fluctuations which affects the signal acquired. A significant detriment in the high frequencies transmission capability is obtained. In order to partially recover the high frequencies information lost, the insertion of a pupil plane filter in the microscope illumination light pathway on the objective lens is proposed. We demonstrate high-frequency and resolution enhancement in the case of linear and non linear fluorescence microscope approach under shot-noise condition.
|Titolo:||Annular pupil filter under shot-noise condition for linear and non linear microscopy|
|Autori interni:||RONZITTI, EMILIANO (Primo)|
|Settore Scientifico Disciplinare:||Settore FIS/03 - Fisica della Materia|
|Data di pubblicazione:||13-apr-2009|
|Digital Object Identifier (DOI):||10.1364/OE.17.006867|
|Appare nelle tipologie:||01 - Articolo su periodico|