Viral factors, such as high‑risk human papillomavirus variants, can increase the risk of viral persistence and influence the progression to cancer. In the present study, the long control region (LCR ) of human papillomavirus (HPV)‑16 and HPV‑52, and the L1 region of HPV‑16 and HPV‑18, identified from subjects belonging to both general and high‑risk populations (migrants, HIV+ subjects and adolescent/young people) residing in Italy, were characterized using molecular and phylogenetic techniques. To the best of our knowledge, this is the first Italian study to analyze a large number of sequences (n=458) and report phylogenetic data on the HPV‑52 variants. The phylogenetic analysis showed that 90% of the LCR variants of HPV‑16 and HPV‑52 clustered within lineage A (European lineage) and only sequences identified from subjects belonging to high‑risk populations fell into the non‑European lineages. Analysis of the LCRs revealed a high genomic diversity with a large number of changes. Several mutations in the binding sites for viral and cellular transcription factors characterized the HPV‑16 LCR variants belonging to the African lineages B and C, were observed in subjects with cytological abnormalities (high squamous intraepithelial lesions). The HPV‑16 and HPV‑18 L1 molecular characterization identified 30% of changes in the immune‑dominant epitope loops. These data give a clear picture of the situation in Italy, and a starting point for understanding the molecular pathogenesis and developing molecular diagnostics for HPV, vaccines and other therapeutic approaches in order to control and/or eliminate virus‑induced diseases.
Genetic characterization of variants of HPV‑16, HPV‑18 and HPV‑52 circulating in Italy among general and high‑risk populations / E.R. Frati, S. Bianchi, A. Amendola, D. Colzani, F. Petrelli, G. Zehender, E. Tanzi. - In: MOLECULAR MEDICINE REPORTS. - ISSN 1791-3004. - 21(2020 Feb), pp. 894-902.
|Titolo:||Genetic characterization of variants of HPV‑16, HPV‑18 and HPV‑52 circulating in Italy among general and high‑risk populations|
FRATI, ELENA ROSANNA (Co-primo)
BIANCHI, SILVIA (Co-primo)
ZEHENDER, GIANGUGLIELMO (Penultimo)
TANZI, ELISABETTA (Ultimo) (Corresponding)
|Parole Chiave:||human papillomavirus type 16, human papillomavirus type 18, human papillomavirus type 52, human papillomavirus variants, genetic variability, single nucleotide polymorphisms|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Data di pubblicazione:||feb-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3892/mmr.2019.10847|
|Appare nelle tipologie:||01 - Articolo su periodico|