Oocytes collected from prepubertal calves lack developmental competence due to their failure or inability to complete ooplasmic maturation. In this study, in order to improve this competence, we transplanted nuclei isolated from GV stage calf oocytes in enucleated matured competent bovine oocytes and we demonstrated that our nuclear technique transplantation is highly effective in reconstituting calf oocyte at a rate of 76.34% and that the reconstructed oocyte can retain its ability to mature in vitro and to be normaly fertilized. The reduced rate of cleavage may be due to denuding required for the micromanipulation step, so, oocytes that receive a transferred GV must undergo the final stages of maturation and the early embryonic development without contacts with their cumulus cells.

Reconstruction of calf oocytes by germinal vesicle transfer in mature bovine oocytes : preliminary results / A. Lange Consiglio, A. Bignotti, A. Pecile, F. Cremonesi. - In: VETERINARY RESEARCH COMMUNICATIONS. - ISSN 0165-7380. - 33:Suppl. 1(2009 Sep), pp. 89-92. ((Intervento presentato al 62. convegno Convegno nazionale tenutosi a San Benedetto del Tronto nel 2008.

Reconstruction of calf oocytes by germinal vesicle transfer in mature bovine oocytes : preliminary results

A. Lange Consiglio
Primo
;
A. Pecile
Penultimo
;
F. Cremonesi
Ultimo
2009

Abstract

Oocytes collected from prepubertal calves lack developmental competence due to their failure or inability to complete ooplasmic maturation. In this study, in order to improve this competence, we transplanted nuclei isolated from GV stage calf oocytes in enucleated matured competent bovine oocytes and we demonstrated that our nuclear technique transplantation is highly effective in reconstituting calf oocyte at a rate of 76.34% and that the reconstructed oocyte can retain its ability to mature in vitro and to be normaly fertilized. The reduced rate of cleavage may be due to denuding required for the micromanipulation step, so, oocytes that receive a transferred GV must undergo the final stages of maturation and the early embryonic development without contacts with their cumulus cells.
Calf oocytes; Cytoplasmic competence; Germinal vesicle transfer
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
Settore VET/01 - Anatomia degli Animali Domestici
Società Italiana delle Scienze Veterinarie
SISVet
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/69696
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