Bovine viral diarrhea virus (BVDV) has been detected in peripheral blood mononuclear cells (PBMCs) of immunocompetent animals, not being clear whether the development of a specific humoral immune response can prevent BVDV infection. The aim of this study was to evaluate the ability of non-cytopathic BVDV to replicate and produce infectious virus in PBMCs from calves pre-infected with BVDV and to elucidate the immunomodulatory effect of BVDV on these cells in an in vitro model. Quantification of virus was by quantitative PCR, while its replicative capacity and shedding into the extracellular environment was evaluated by viral titration. Apoptosis was assessed by flow cytometry analysis of annexin V and propidium iodide, and by expression of caspase-3/7. Flow cytometry was used to analyze the expression of CD14/CD11b/CD80, CD4/CD8/CD25, MHC-I/MHC-II and B-B2 markers. Our results showed that PBMCs from cattle naturally infected with BVDV were more susceptible to in vitro BVDV infection and showed a more severe apoptosis response than those from naïve animals. Non-cytopathic BVDV in vitro infection also resulted in a lack of effect in the expression of antigen presentation surface markers. All these findings could be related to the immunosuppressive capacity of BVDV and the susceptibility of cattle to this infection.
BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV / M.A. Risalde, F. Romero-Palomo, C. Lecchi, F. Ceciliani, C. Bazzocchi, S. Comazzi, M. Besozzi, J.C. Gómez-Villamandos, C. Luzzago. - In: COMPARATIVE IMMUNOLOGY, MICROBIOLOGY AND INFECTIOUS DISEASES. - ISSN 0147-9571. - 68(2020 Feb).
|Titolo:||BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV|
LUZZAGO, CAMILLA (Ultimo)
|Parole Chiave:||Apoptosis; Bovine viral diarrhea virus; Immunological surface markers expression; Peripheral blood mononuclear cells; Virus titration|
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
Settore VET/05 - Malattie Infettive degli Animali Domestici
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
|Data di pubblicazione:||feb-2020|
|Data ahead of print / Data di stampa:||2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.cimid.2019.101388|
|Appare nelle tipologie:||01 - Articolo su periodico|