Despite the increasing availability of feline blood, which is collected and stored for transfusion purposes, few studies have assessed the effect of storage on feline whole blood (WB) units. The purpose of this study was to investigate selected hematologic and biochemical changes during storage of feline WB units and to determine when they occurred. Data from a quality control program for WB units was used in this study. Twelve feline WB units, collected using an open system, were sampled every 7 days from the point of collection to the end of storage at 35 days (D0, D7, D14, D21, D28, and D35). Measurements at each time point were: (1) hematologic parameters; (2) percentage hemolysis; (3) morphologic index scored at 0–3, based on echinocyte transformation of the erythrocytes; and (4) selected biochemical parameters. Aerobic and anaerobic culture was performed at D0 and D35. Results were compared statistically to D0 (statistical significance set at <0.01). Storage did not result in statistically significant changes in measured hematological parameters. There were statistically significant increases in percentage hemolysis and morphologic index, starting from D21 (P = 0.000 and P = 0.004, respectively). Glucose decreased significantly from D21 (P = 0.003); potassium increased significantly from D7 (P=0.001); and sodium increased significantly, starting from D28 (P = 0.009). Bacteria were not isolated. Blood in feline WB units collected using an open system underwent some significant storage changes that were time-dependent. As these changes could affect the quality and the utility of stored WB used in feline transfusion medicine, further study is required to determine their clinical importance.

Hematological, biochemical and microbiological evaluation of feline whole blood units collected using an open system and stored for 35 days / E. Spada, R. Perego, L. Baggiani, P.A. Martino, D. Proverbio. - In: THE VETERINARY JOURNAL. - ISSN 1090-0233. - 254(2019 Dec). [10.1016/j.tvjl.2019.105396]

Hematological, biochemical and microbiological evaluation of feline whole blood units collected using an open system and stored for 35 days

E. Spada
Primo
Conceptualization
;
R. Perego
Secondo
;
L. Baggiani;P.A. Martino
Penultimo
;
D. Proverbio
Ultimo
2019-12

Abstract

Despite the increasing availability of feline blood, which is collected and stored for transfusion purposes, few studies have assessed the effect of storage on feline whole blood (WB) units. The purpose of this study was to investigate selected hematologic and biochemical changes during storage of feline WB units and to determine when they occurred. Data from a quality control program for WB units was used in this study. Twelve feline WB units, collected using an open system, were sampled every 7 days from the point of collection to the end of storage at 35 days (D0, D7, D14, D21, D28, and D35). Measurements at each time point were: (1) hematologic parameters; (2) percentage hemolysis; (3) morphologic index scored at 0–3, based on echinocyte transformation of the erythrocytes; and (4) selected biochemical parameters. Aerobic and anaerobic culture was performed at D0 and D35. Results were compared statistically to D0 (statistical significance set at <0.01). Storage did not result in statistically significant changes in measured hematological parameters. There were statistically significant increases in percentage hemolysis and morphologic index, starting from D21 (P = 0.000 and P = 0.004, respectively). Glucose decreased significantly from D21 (P = 0.003); potassium increased significantly from D7 (P=0.001); and sodium increased significantly, starting from D28 (P = 0.009). Bacteria were not isolated. Blood in feline WB units collected using an open system underwent some significant storage changes that were time-dependent. As these changes could affect the quality and the utility of stored WB used in feline transfusion medicine, further study is required to determine their clinical importance.
Feline; Hemolysis; RBC morphology; Storage lesion; Whole blood unit
Settore VET/08 - Clinica Medica Veterinaria
PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 2 "DOTAZIONE ANNUALE PER ATTIVITA' ISTITUZIONALE"
Fondo per il finanziamento delle attività base di ricerca - DIPARTIMENTO DI SCIENZE VETERINARIE PER LA SALUTE, LA PRODUZIONE ANIMALE E LA SICUREZZA ALIMENTARE
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/687642
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