The REproductive Meristem (REM) gene family encodes for transcription factors belonging to the B3 DNA binding domain superfamily. In Arabidopsis thaliana the REM gene family is composed of 45 members, preferentially expressed during flower, ovule and seed development. Only a few members of this family have been functionally characterized: VERNALIZATION1 (VRN1) and most recently TARGET OF FLC AND SVP1 (TFS1) regulate flowering time and VERDANDI (VDD), together with VALKYRIE (VAL) control the death of the receptive synergid cell in the female gametophyte. We investigated the role of REM34, REM35 and REM36, three closely related and linked genes similarly expressed in both female and male gametophytes. Simultaneous silencing by RNA interference (RNAi) caused about 50% of the ovules to remain unfertilized. Careful evaluation of both ovule and pollen development showed that this partial sterility of the transgenic RNAi lines was due to a post meiotic block in both female and male gametophytes. Furthermore, protein interaction assays revealed that REM34 and REM35 interact, which suggests that they work together during the first stages of gametogenesis.

REM34 and REM35 control female and male gametophyte development in Arabidopsis thaliana / F. Caselli, V. Beretta, O. Mantegazza, R. Petrella, G. Leo, A. Guazzotti, H. Herrera-Ubaldo, S. de Folter, M. Mendes, M. Kater, V. Gregis. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 10(2019 Oct 24). [10.3389/fpls.2019.01351]

REM34 and REM35 control female and male gametophyte development in Arabidopsis thaliana

F. Caselli
Primo
;
V. Beretta
Secondo
;
O. Mantegazza;R. Petrella;G. Leo;A. Guazzotti;M. Mendes;M. Kater;V. Gregis
Ultimo
2019

Abstract

The REproductive Meristem (REM) gene family encodes for transcription factors belonging to the B3 DNA binding domain superfamily. In Arabidopsis thaliana the REM gene family is composed of 45 members, preferentially expressed during flower, ovule and seed development. Only a few members of this family have been functionally characterized: VERNALIZATION1 (VRN1) and most recently TARGET OF FLC AND SVP1 (TFS1) regulate flowering time and VERDANDI (VDD), together with VALKYRIE (VAL) control the death of the receptive synergid cell in the female gametophyte. We investigated the role of REM34, REM35 and REM36, three closely related and linked genes similarly expressed in both female and male gametophytes. Simultaneous silencing by RNA interference (RNAi) caused about 50% of the ovules to remain unfertilized. Careful evaluation of both ovule and pollen development showed that this partial sterility of the transgenic RNAi lines was due to a post meiotic block in both female and male gametophytes. Furthermore, protein interaction assays revealed that REM34 and REM35 interact, which suggests that they work together during the first stages of gametogenesis.
Gametophyte development, Ovule, Pollen, post-meiotic division, Transcriptional regulation, REMS, Arabidopsis thaliana
Settore BIO/18 - Genetica
Settore BIO/01 - Botanica Generale
   MADS box transcription factor SHORT VEGETATIVE PHASE, understanding the molecular mechanism and involvement in sense and antisense transcript regulation
   MADSMEC
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   RBSI14BTZR

   Exploring the molecular control of seed yield in crops
   ExpoSEED
   EUROPEAN COMMISSION
   H2020
   691109
24-ott-2019
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/684970
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