The nucleolus, the site of pre-ribosomal RNA (pre-rRNA) synthesis and processing in eukaryotic cells, contains a number of small nucleolar RNAs (snoRNAs). Yeast U3 snoRNA is required for the processing of 18S rRNA from larger precursors and contains a region complementary to the pre-rRNA. Substitution mutations in the pre-rRNA which disrupt this base pairing potential are lethal and prevent synthesis of 18S rRNA. These mutant pre-rRNAs show defects in processing which closely resemble the effects of genetic depletion of components of the U3 snoRNP. Co-expression of U3 snoRNAs which carry compensatory mutations allows the mutant pre-rRNAs to support viability and synthesize 18S rRNA at high levels. Pre-rRNA processing steps which are blocked by the external transcribed spacer region mutations are largely restored by expression of the compensatory U3 mutants. Pre-rRNA processing therefore requires direct base pairing between snoRNA and the substrate. Base pairing with the substrate is thus a common feature of small RNAs involved in mRNA and rRNA maturation.
Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis / M. Beltrame, D. Tollervey. - In: EMBO JOURNAL. - ISSN 0261-4189. - 14:17(1995 Sep 01), pp. 4350-4356.
|Titolo:||Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis|
BELTRAME, MONICA DANIELA (Corresponding)
|Parole Chiave:||RNA; RNA processing; ribosomes; small necleolar RNAS; U3|
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
Settore BIO/11 - Biologia Molecolare
|Data di pubblicazione:||1-set-1995|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/j.1460-2075.1995.tb00109.x|
|Appare nelle tipologie:||01 - Articolo su periodico|