Interactions between proteins are essential for their functioning and the biological processes they control. The elucidation of interaction maps based on yeast studies is a first step toward the understanding of molecular networks and provides a framework of proteins that possess the capacity and specificity to interact. Here, we present a comprehensive plant protein-protein interactome map of nearly all members of the Arabidopsis thaliana MADS box transcription factor family. A matrix-based yeast two-hybrid screen of >100 members of this family revealed a collection of specific heterodimers and a few homodimers. Clustering of proteins with similar interaction patterns pinpoints proteins involved in the same developmental program and provides valuable information about the participation of uncharacterized proteins in these programs. Furthermore, a model is proposed that integrates the floral induction and floral organ formation networks based on the interactions between the proteins involved. Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower.
|Titolo:||Comprehensive Interaction Map of the Arabidopsis MADS Box Transcription Factors|
|Autori interni:||COLOMBO, LUCIA|
|Parole Chiave:||Protein interaction ; Dimerization ; Morphogenesis ; Flowers ; Transcription factors ; Arabidopsis thaliana|
|Settore Scientifico Disciplinare:||Settore BIO/01 - Botanica Generale|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1105/tpc.105.031831|
|Appare nelle tipologie:||01 - Articolo su periodico|