X-linked inhibitor of apoptosis protein (XIAP) is an emerging crucial therapeutic target in cancer. We report on the discovery and characterisation of small organic molecules from Piper genus plants exhibiting XIAP antagonism, namely erioquinol, a quinol substituted in the 4-position with an alkenyl group and the alkenylphenols eriopodols A-C. Another isolated compound was originally identified as gibbilimbol B. Erioquinol was the most potent inhibitor of human cancer cell viability when compared with gibbilimbol B and eriopodol A was listed as intermediate. Gibbilimbol B and eriopodol A induced apoptosis through mitochondrial permeabilisation and caspase activation while erioquinol acted on cell fate via caspase-independent/non-apoptotic mechanisms, likely involving mitochondrial dysfunctions and aberrant generation of reactive oxygen species. In silico modelling and molecular approaches suggested that all molecules inhibit XIAP by binding to XIAP-baculoviral IAP repeat domain. This demonstrates a novel aspect of XIAP as a key determinant of tumour control, at the molecular crossroad of caspase-dependent/independent cell death pathway and indicates molecular aspects to develop tumour-effective XIAP antagonists.

XIAP as a Target of New Small Organic Natural Molecules Inducing Human Cancer Cell Death / D. Muñoz, M. Brucoli, S. Zecchini, A. Sandoval-Hernandez, G. Arboleda, F. Lopez-Vallejo, W. Delgado, M. Giovarelli, M. Coazzoli, E. Catalani, C. De Palma, C. Perrotta, L. Cuca, E. Clementi, D. Cervia. - In: CANCERS. - ISSN 2072-6694. - 11:9(2019 Sep). [10.3390/cancers11091336]

XIAP as a Target of New Small Organic Natural Molecules Inducing Human Cancer Cell Death

S. Zecchini;M. Giovarelli;M. Coazzoli;C. De Palma;C. Perrotta;E. Clementi
Penultimo
;
2019

Abstract

X-linked inhibitor of apoptosis protein (XIAP) is an emerging crucial therapeutic target in cancer. We report on the discovery and characterisation of small organic molecules from Piper genus plants exhibiting XIAP antagonism, namely erioquinol, a quinol substituted in the 4-position with an alkenyl group and the alkenylphenols eriopodols A-C. Another isolated compound was originally identified as gibbilimbol B. Erioquinol was the most potent inhibitor of human cancer cell viability when compared with gibbilimbol B and eriopodol A was listed as intermediate. Gibbilimbol B and eriopodol A induced apoptosis through mitochondrial permeabilisation and caspase activation while erioquinol acted on cell fate via caspase-independent/non-apoptotic mechanisms, likely involving mitochondrial dysfunctions and aberrant generation of reactive oxygen species. In silico modelling and molecular approaches suggested that all molecules inhibit XIAP by binding to XIAP-baculoviral IAP repeat domain. This demonstrates a novel aspect of XIAP as a key determinant of tumour control, at the molecular crossroad of caspase-dependent/independent cell death pathway and indicates molecular aspects to develop tumour-effective XIAP antagonists.
Piper eriopodon, alkenylphenols; XIAP antagonists; XIAP-BIR3 domain; apoptosis; caspase-independent cell death; cell death; human cancer cells; phytochemicals; small organic agents
Settore BIO/14 - Farmacologia
set-2019
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/677800
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