In this study, hybrid nanocubes composed of magnetite (Fe3O4) and manganese dioxide (MnO2), coated with U-251 MG cell-derived membranes (CM-NCubes) are synthesized. The CM-NCubes demonstrate a concentration-dependent oxygen generation (up to 15%), and, for the first time in the literature, an intracellular increase of temperature (6 °C) due to the exothermic scavenging reaction of hydrogen peroxide (H2O2) is showed. Internalization studies demonstrate that the CM-NCubes are internalized much faster and at a higher extent by the homotypic U-251 MG cell line compared to other cerebral cell lines. The ability of the CM-NCubes to cross an in vitro model of the blood-brain barrier is also assessed. The CM-NCubes show the ability to respond to a static magnet and to accumulate in cells even under flowing conditions. Moreover, it is demonstrated that 500 µg mL−1 of sorafenib-loaded or unloaded CM-NCubes are able to induce cell death by apoptosis in U-251 MG spheroids that are used as a tumor model, after their exposure to an alternating magnetic field (AMF). Finally, it is shown that the combination of sorafenib and AMF induces a higher enzymatic activity of caspase 3 and caspase 9, probably due to an increment in reactive oxygen species by means of hyperthermia.
Cell Membrane-Coated Magnetic Nanocubes with a Homotypic Targeting Ability Increase Intracellular Temperature due to ROS Scavenging and Act as a Versatile Theranostic System for Glioblastoma Multiforme / C. Tapeinos, F. Tomatis, M. Battaglini, A. Larranaga, A. Marino, I.A. Telleria, M. Angelakeris, D. Debellis, F. Drago, F. Brero, P. Arosio, A. Lascialfari, A. Petretto, E. Sinibaldi, G. Ciofani. - In: ADVANCED HEALTHCARE MATERIALS. - ISSN 2192-2659. - 8:18(2019 Sep).
|Titolo:||Cell Membrane-Coated Magnetic Nanocubes with a Homotypic Targeting Ability Increase Intracellular Temperature due to ROS Scavenging and Act as a Versatile Theranostic System for Glioblastoma Multiforme|
|Parole Chiave:||cell membrane-coated nanocubes; glioblastoma; homotypic targeting; magnetic hyperthermia; theranostics|
|Settore Scientifico Disciplinare:||Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)|
|Data di pubblicazione:||set-2019|
|Data ahead of print / Data di stampa:||ago-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/adhm.201900612|
|Appare nelle tipologie:||01 - Articolo su periodico|
File in questo prodotto:
|51_Cell membrane nanocubes_AdvHealthcareMater_2019.pdf||Publisher's version/PDF||Administrator Richiedi una copia|
|EMS83997.pdf||Post-print, accepted manuscript ecc. (versione accettata dall'editore)||Open Access Visualizza/Apri|