Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an α-subunit (αGltS) and a β-subunit (βGltS) that assemble in different αβ oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (α4β3, α4β4, α6β4 and α6β6, in the 3.5-4.1 Å resolution range). Our study provides a comprehensive GltS model, which details the inter-protomeric assemblies, allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S]+1,+2 clusters within βGltS.

Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in its Oligomeric Assemblies / P. Swuec, A. Chaves-Sanjuan, C. Camilloni, M.A. Vanoni, M. Bolognesi. - In: JOURNAL OF MOLECULAR BIOLOGY. - ISSN 0022-2836. - 431:22(2019 Nov), pp. 4523-4526. [10.1016/j.jmb.2019.08.011]

Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in its Oligomeric Assemblies

P. Swuec
Primo
;
A. Chaves-Sanjuan
Secondo
;
C. Camilloni;M.A. Vanoni
Penultimo
;
M. Bolognesi
Ultimo
2019

Abstract

Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an α-subunit (αGltS) and a β-subunit (βGltS) that assemble in different αβ oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (α4β3, α4β4, α6β4 and α6β6, in the 3.5-4.1 Å resolution range). Our study provides a comprehensive GltS model, which details the inter-protomeric assemblies, allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S]+1,+2 clusters within βGltS.
Cryo electron microscopy; Fe/S clusters; Glutamate synthase; Iron/sulfur flavoprotein; Protein oligomeric structure
Settore BIO/10 - Biochimica
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
nov-2019
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/674765
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