Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren's syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 subjects, including in SLE (n = 27), SjS (n = 23), PAPs (n = 11) and undifferentiated connective tissue (UCTD, n = 26) patients, and geographically-matched healthy controls (HCs, n = 27), were collected for microbiome (16s rRNA gene sequencing) and metabolome (high-performance liquid chromatography coupled to mass spectrometry) analysis to identify shared characteristics across diseases. Out of 130 identified microbial genera, a subset of 29 bacteria was able to differentiate study groups (area under receiver operating characteristics (AUROC) = 0.730 ± 0.025). A fair classification was obtained with a subset of 41 metabolic peaks out of 254 (AUROC = 0.748 ± 0.021). In both models, HCs were well separated from SADs, while UCTD largely overlapped with the other diseases. In all of the SADs pro-tolerogenic bacteria were reduced, while pathobiont genera were increased. Metabolic alterations included two clusters comprised of: (a) members of the acylcarnitine family, positively correlating with a Prevotella-enriched cluster and negatively correlating with a butyrate-producing bacteria-enriched cluster; and (b) phospholipids, negatively correlating with butyrate-producing bacteria. These findings demonstrate a strong interaction between intestinal microbiota and metabolic function in patients with SADs.
Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases / C. Bellocchi, Á. Fernández-Ochoa, G. Montanelli, B. Vigone, A. Santaniello, R. Quirantes-Piné, I. Borrás-Linares, M. Gerosa, C. Artusi, R. Gualtierotti, A. Segura-Carrettero, M.E. Alarcón-Riquelme, L. Beretta. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 8:9(2019 Aug 23), pp. 1291.1-1291.15.
|Titolo:||Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases|
|Parole Chiave:||Sjögren’s syndrome; metabolomics; microbiomic; primary anti-phosholipid syndrome; systemic autoimmune diseases; systemic lupus erythematosus; undifferentiated connective tissue diseases|
|Settore Scientifico Disciplinare:||Settore MED/16 - Reumatologia|
|Progetto:||Molecular Reclassification to Find Clinically Useful Biomarkers for Systemic Autoimmune Diseases|
|Data di pubblicazione:||23-ago-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/jcm8091291|
|Appare nelle tipologie:||01 - Articolo su periodico|