IL-1R8 is a member of Interleukin-1 receptor family acing as a negative regulator of inflammation reliant on ILRs and TLRs activation. IL-1R8 role has never been evaluated in acute bacterial mastitis. We first investigated IL-1R8 sequence conservation among different species and its pattern of expression in a wide panel of organs from healthy goats. Then, modulation of IL-1R8 during natural and experimental mammary infection was evaluated and compared in blood, milk and mammary issues from healthy and Staphylococcus aureus infected goats. IL-1R8 has a highly conserved sequence among vertebrates. Goat IL-1R8 was ubiquitously expressed in epithelial and lymphoid issues with highest levels in pancreas. IL-1R8 was down-regulated in epithelial mammary cells following S. aureus infection. Interestingly it was up-regulated in leukocytes infiltrating the infected mammary issues suggesting that it could represent a target of S. aureus immune evasion.
Staphylococcus aureus intra-mammary infection affects the expression pattern of IL-R8 in goat / J. Filipe, V. Bronzo, G. Curone, B. Castiglioni, D. Vigo, B. Smith, V. Herrera Millar, P. Roccabianca, P. Moroni, F. Riva. - In: COMPARATIVE IMMUNOLOGY, MICROBIOLOGY AND INFECTIOUS DISEASES. - ISSN 0147-9571. - 66(2019 Oct).
Staphylococcus aureus intra-mammary infection affects the expression pattern of IL-R8 in goat
J. Filipe;V. Bronzo;G. Curone;D. Vigo;V. Herrera Millar;P. Roccabianca;P. Moroni;F. Riva
2019
Abstract
IL-1R8 is a member of Interleukin-1 receptor family acing as a negative regulator of inflammation reliant on ILRs and TLRs activation. IL-1R8 role has never been evaluated in acute bacterial mastitis. We first investigated IL-1R8 sequence conservation among different species and its pattern of expression in a wide panel of organs from healthy goats. Then, modulation of IL-1R8 during natural and experimental mammary infection was evaluated and compared in blood, milk and mammary issues from healthy and Staphylococcus aureus infected goats. IL-1R8 has a highly conserved sequence among vertebrates. Goat IL-1R8 was ubiquitously expressed in epithelial and lymphoid issues with highest levels in pancreas. IL-1R8 was down-regulated in epithelial mammary cells following S. aureus infection. Interestingly it was up-regulated in leukocytes infiltrating the infected mammary issues suggesting that it could represent a target of S. aureus immune evasion.
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