Background: Cell blocks and immunohistochemistry (IHC) are increasingly recognized as being complementary tools for cytologic diagnostics, especially for neoplastic diseases. Objectives: The study aimed to evaluate the utility of cell tube block (CTB) IHC for refining the diagnosis of effusions in dogs and cats. Methods: Cavitary effusions (n = 25) from dogs and cats classified by cytology as reactive, neoplastic, borderline (suspicious of neoplasia), and chylous were studied. CTB sections were stained with H&E, and immunostained with PAX-5, CD3, pancytokeratin (CK), vimentin, and Wilms tumor 1 protein (WT1) antibodies, according to the cytologic diagnoses. A histologic case series of confirmed normal, reactive, and neoplastic mesothelium and several different carcinomas were included to test the utility of WT1 as a marker of mesothelial cells. Results: CTBs had a layered appearance with reduced background staining. CD3 and PAX5 immunolabeling allowed immunophenotype assessment in all of the lymphoma cases. In carcinomatous effusions, neoplastic cells were CK-positive, WT1-negative, and vimentin-negative (except for two cases). Wilms tumor 1 protein was positive in the nuclei of normal, reactive, and neoplastic mesothelial cells, and ovarian carcinomatous cells. Other carcinomas and lymphomas were negative. Conclusions: CTBs are valuable tools to assist in making a diagnosis of cavitary effusions in dogs and cats, and WT1 is a promising marker to differentiate mesothelial from carcinomatous cells.
The cell tube block technique and an immunohistochemistry panel including Wilms tumor 1 to assist in diagnosing cavitary effusions in dogs and cats / R. Marcos, C. Marrinhas, F. Malhao, A. Canadas, M. Santos, M. Caniatti. - In: VETERINARY CLINICAL PATHOLOGY. - ISSN 0275-6382. - 48:1(2019 Mar), pp. 50-60.
|Titolo:||The cell tube block technique and an immunohistochemistry panel including Wilms tumor 1 to assist in diagnosing cavitary effusions in dogs and cats|
CANIATTI, MARIO (Ultimo) [Supervision]
|Parole Chiave:||cell blocks; cytology; effusion; immunohistochemistry; mesothelial cells; Animals; Ascitic Fluid; Cat Diseases; Cats; Dog Diseases; Dogs; Histocytological Preparation Techniques; Immunohistochemistry; Kidney Neoplasms; Pericardial Effusion; Pleural Effusion; Wilms Tumor|
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Data di pubblicazione:||mar-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/vcp.12709|
|Appare nelle tipologie:||01 - Articolo su periodico|
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