The pancreatic lymph node is critical to the pathogenesis of autoimmune diabetes, as it constitutes the initial site for the priming of autoreactive T cells. In this study, we compared the histopathology of the head pancreatic lymph node (HPLN) to the tail pancreatic lymph node (TPLN) in NOD mice. HPLNs and TPLNs were harvested from 4 week-, 8 week-, and 12 week-old NOD mice, and their microvasculature, extracellular matrix, and immune cell subsets were characterized. The percentages of B cells and antigen-presenting cells (APCs) were much higher in the HPLN, as compared to the TPLN. Notably, the HPLNs of 12 week-old mice were characterized by greater expansion of high endothelial venules (HEVs) and lymphatic vessels in comparison to the TPLNs. Finally, we observed a higher density of extracellular matrix (ECM) fibers surrounding the lymphatic vasculature in the HPLNs than in the TPLNs. These data for the first time demonstrate that the HPLN possesses a different immune microanatomy and organization from the TPLN. These novel observations unveil a major phenotypic difference between two types of LNs from the same organ and may highlight an independent fundamental role played by each PLN during the establishment of T1D.
Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice / X. Li, A. Bean, M. Uehara, N. Banouni, M. Ben Nasr, V. Kasinath, L. Jiang, P. Fiorina, R. Abdi. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 9:1(2019), pp. 9778.1-9778.7.
|Titolo:||Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice|
FIORINA, PAOLO (Penultimo)
|Settore Scientifico Disciplinare:||Settore MED/13 - Endocrinologia|
|Data di pubblicazione:||2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41598-019-45899-1|
|Appare nelle tipologie:||01 - Articolo su periodico|