Background: Inflammatory bowel disease (IBD) is thought to be due to an abnormal interaction between the host immune system and commensal microflora. Within the intestinal immune system, B cells produce physiologically natural antibodies but pathologically atypical anti-neutrophil antibodies (xANCAs) are frequently observed in patients with IBD. The objective is to investigate the localisation of immunoglobulin-producing cells (IPCs) in samples of inflamed intestinal tissue taken from patients with IBD, and their possible relationship with clinical features.Methods: The IPCs in small intestinal, colonic and rectal biopsy specimens of patients with IBD were analysed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The B cell phenotype of the IPC-positive samples was assessed using monoclonal antibodies specific for CD79, CD20, CD23, CD21, CD5, λ and κ chains. Statistical correlations were sought between the histological findings and clinical expression.Results: The study involved 96 patients (64 with ulcerative colitis and 32 with Crohn's disease). Two different patterns of B lymphocyte infiltrates were found in the intestinal tissue: one was characterised by a strong to moderate stromal localisation of small IgM +/CD79 +/CD20 -/CD21 -/CD23 -/CD5 ± IPCs (42.7% of cases); in the other (57.3%) no such small IPCs were detected in stromal or epithelial tissues. IPCs were significantly less frequent in the patients with Crohn's disease than in those with ulcerative colitis (p = 0.004).Conclusion: Our findings suggest that different immunopathogenetic pathways underlie chronic intestinal inflammation with different clinical expressions. The presence of small B lymphocytes resembling B-1 cells also seemed to be negatively associated with Crohn's disease. It can therefore be inferred that the gut contains an alternative population of B cells that have a regulatory function.

B Lymphocyte intestinal homing in inflammatory bowel disease / C. Defendenti, P. Sarzi-Puttini, S. Grosso, A. Croce, O. Senesi, S. Saibeni, S. Bollani, P.L. Almasio, S. Bruno, F. Atzeni. - In: BMC IMMUNOLOGY. - ISSN 1471-2172. - 12:1(2011 Dec), pp. 71.1-71.6.

B Lymphocyte intestinal homing in inflammatory bowel disease

P. Sarzi-Puttini;S. Saibeni;S. Bollani;
2011

Abstract

Background: Inflammatory bowel disease (IBD) is thought to be due to an abnormal interaction between the host immune system and commensal microflora. Within the intestinal immune system, B cells produce physiologically natural antibodies but pathologically atypical anti-neutrophil antibodies (xANCAs) are frequently observed in patients with IBD. The objective is to investigate the localisation of immunoglobulin-producing cells (IPCs) in samples of inflamed intestinal tissue taken from patients with IBD, and their possible relationship with clinical features.Methods: The IPCs in small intestinal, colonic and rectal biopsy specimens of patients with IBD were analysed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The B cell phenotype of the IPC-positive samples was assessed using monoclonal antibodies specific for CD79, CD20, CD23, CD21, CD5, λ and κ chains. Statistical correlations were sought between the histological findings and clinical expression.Results: The study involved 96 patients (64 with ulcerative colitis and 32 with Crohn's disease). Two different patterns of B lymphocyte infiltrates were found in the intestinal tissue: one was characterised by a strong to moderate stromal localisation of small IgM +/CD79 +/CD20 -/CD21 -/CD23 -/CD5 ± IPCs (42.7% of cases); in the other (57.3%) no such small IPCs were detected in stromal or epithelial tissues. IPCs were significantly less frequent in the patients with Crohn's disease than in those with ulcerative colitis (p = 0.004).Conclusion: Our findings suggest that different immunopathogenetic pathways underlie chronic intestinal inflammation with different clinical expressions. The presence of small B lymphocytes resembling B-1 cells also seemed to be negatively associated with Crohn's disease. It can therefore be inferred that the gut contains an alternative population of B cells that have a regulatory function.
B1 cells; Inflammation; Inflammatory bowel disease; Lymphocyte homing; Lymphocytes; Mucosal immunity; Adult; Aged; Antigens, CD; B-Lymphocytes; Biopsy; Cell Differentiation; Cell Movement; Female; Fluorescent Antibody Technique; Humans; Immunoglobulin M; Immunomodulation; Immunophenotyping; Inflammatory Bowel Diseases; Intestinal Mucosa; Intestines; Male; Middle Aged
Settore MED/16 - Reumatologia
dic-2011
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/667533
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