4-Aminobiphenyl (4-ABP)-DNA adducts and p53 overexpression were evaluated in laryngeal biopsies from 38 patients by immunohistochemical methods. Samples were categorized as tumors (n = 9), polyps (n = 28) or normal tissue (n = 1). 4-BBP-DNA adducts were evaluated with a quantitative immunoperoxidase method using monoclonal antibody 3C8 in both the lesion and adjacent tissue. Relative staining intensity data showed a log-normal distribution and values found in adjacent tissue from smokers were significantly higher (median: 173.5, geometric mean: 159.9) than those measured in adjacent tissue from non-smokers (median: 75.5, geometric mean: 7.40). Statistical significance was assessed both by non-parametric testing on raw data (P = 0.0007 on rank sum test) and by parametric testing on log-transformed data (P = 0.0002 on an unpaired t-test). Furthermore, relative staining intensity in the lesional tissue showed the same significant difference between smokers and non-smokers in patients affected by polyps, whereas no significant difference was detected in patients with laryngeal tumors. Overexpression of p53, also measured with an immunoperoxidase method, was observed in 44% of the malignant tumors and in 3.5% of the polyps. This work demonstrates that 4-ABP-DNA adducts can be evaluated in laryngeal tissue and are related to smoking exposure.
4-Aminobiphenyl-DNA adducts in laryngeal tissue and smoking habits: an immunohistochemical study / G. Flamini, G. Romano, G. Curigliano, A. Chiominto, G. Capelli, A. Boninsegna, C. Signorelli, L. Ventura, R.M. Santella, A. Sgambato, A. Cittadini. - In: CARCINOGENESIS. - ISSN 0143-3334. - 19:2(1998), pp. 353-357.
|Titolo:||4-Aminobiphenyl-DNA adducts in laryngeal tissue and smoking habits: an immunohistochemical study|
CURIGLIANO, GIUSEPPE (Corresponding)
|Parole Chiave:||Nuclear overexpression; protein overexpression; hemoglobin adducts; cigarette smokers; aromatic-amines; tobacco-smoke; DNA adducts; cancer; P53; nonsmokers|
|Settore Scientifico Disciplinare:||Settore MED/06 - Oncologia Medica|
|Data di pubblicazione:||1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/carcin/19.2.353|
|Appare nelle tipologie:||01 - Articolo su periodico|