Objective. Nonlymphoma-associated bcl-2/IgH rearrangements (NLABRs) are frequently amplified by PCR in blood of lymphoma-free subjects (LFS), but the temporal kinetics and phenotypic nature of NLABR-positive cells are unknown. To address these issues we prospectively monitored a panel of NLABR-positive LFS. Methods. LFS have been studied by nested PCR, real-time PCR, and DNA sequencing. Cell selection studies were also performed to define the nature of NLABR-bearing clones. Results. Of 125 donors, 16 (12.8%) were found to be bcl-2/IgH positive and were monitored at least every 6 months for a median time of 22 months (range 6-50). In half of the subjects the same NLABR detected initially was again reamplified at follow-up thrice or more. In 5, the same NLABR was constantly amplified in every follow-up sample. With a median follow-up of 22 months (range 9-50), no stable disappearance of a recurrent clone has been so far recorded. Real-time PCR indicated that persistent NLABR-positive clones are stable over time in the same subject. Cell separation studies indicate that NLABRs belong to CD19(+), CD5(-), CD23(-), CD10(+/-) cells. Conclusions. Our results indicate that NLABR-positive clones are persistent populations phenotypically related to follicular lymphoma (FL.). This suggests the existence of a FL-related clonal expansion of undetermined significance, which might be either a premalignant or a nonmalignant counterpart of FL.

Cells carrying nonlymphoma-associated bcl-2/IgH rearrangements (NLABR) are phenotypically related to follicular lymphoma and can establish as long-term persisting clonal populations / M. Ladetto, B. Mantoan, F. DE MARCO, D. Drandi, C. Aguzzi, M. Astolfi, S. Vallet, I. Ricca, M. Aquila, G. Pagliano, L. Monitillo, B. Pollio, L. Santo, C. Cristiano, A. Rocci, R. Francese, C. Bodoni, A. Borchiellini, P. Schinco, M. Boccadoro, C. Tarella. - In: EXPERIMENTAL HEMATOLOGY. - ISSN 0301-472X. - 34:12(2006), pp. 1680-1686. [10.1016/j.exphem.2006.08.008]

Cells carrying nonlymphoma-associated bcl-2/IgH rearrangements (NLABR) are phenotypically related to follicular lymphoma and can establish as long-term persisting clonal populations

C. Tarella
2006

Abstract

Objective. Nonlymphoma-associated bcl-2/IgH rearrangements (NLABRs) are frequently amplified by PCR in blood of lymphoma-free subjects (LFS), but the temporal kinetics and phenotypic nature of NLABR-positive cells are unknown. To address these issues we prospectively monitored a panel of NLABR-positive LFS. Methods. LFS have been studied by nested PCR, real-time PCR, and DNA sequencing. Cell selection studies were also performed to define the nature of NLABR-bearing clones. Results. Of 125 donors, 16 (12.8%) were found to be bcl-2/IgH positive and were monitored at least every 6 months for a median time of 22 months (range 6-50). In half of the subjects the same NLABR detected initially was again reamplified at follow-up thrice or more. In 5, the same NLABR was constantly amplified in every follow-up sample. With a median follow-up of 22 months (range 9-50), no stable disappearance of a recurrent clone has been so far recorded. Real-time PCR indicated that persistent NLABR-positive clones are stable over time in the same subject. Cell separation studies indicate that NLABRs belong to CD19(+), CD5(-), CD23(-), CD10(+/-) cells. Conclusions. Our results indicate that NLABR-positive clones are persistent populations phenotypically related to follicular lymphoma (FL.). This suggests the existence of a FL-related clonal expansion of undetermined significance, which might be either a premalignant or a nonmalignant counterpart of FL.
Polymerase-chain-reaction; time quantitative PCR; healthy-individuals; chromosomal translocation; genomic instability; peripheral-blood; multiple-myeloma; residual disease; B-cells; cancer
Settore MED/15 - Malattie del Sangue
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0301472X06005091-main.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 202.24 kB
Formato Adobe PDF
202.24 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/663247
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 10
social impact