The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.
Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state / R. Artali, N. Marchini, F. Meneghetti, D. Cavazzini, A. Cassetta, C. Sassone. - In: ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. - ISSN 0907-4449. - 61:part 4(2005), pp. 481-484. [10.1107/S0907444905001502]
Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state
R. ArtaliPrimo
;F. Meneghetti;
2005
Abstract
The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.Pubblicazioni consigliate
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